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Applied and Environmental Microbiology, November 2007, p. 6757-6767, Vol. 73, No. 21
0099-2240/07/$08.00+0     doi:10.1128/AEM.00637-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Adaptation and Response of Bifidobacterium animalis subsp. lactis to Bile: a Proteomic and Physiological Approach{triangledown}

Borja Sánchez,1,2 Marie-Christine Champomier-Vergès,1* Birgitte Stuer-Lauridsen,3 Patricia Ruas-Madiedo,2 Patricia Anglade,1 Fabienne Baraige,1 Clara G. de los Reyes-Gavilán,2 Eric Johansen,3 Monique Zagorec,1 and Abelardo Margolles2

Unité Flore Lactique et Environnement Carné (UR309), INRA, Domaine de Vilvert, F-78350 Jouy-en-Josas, France,1 Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (CSIC), Ctra. Infiesto s/n, 33300 Villaviciosa, Asturias, Spain,2 Chr. Hansen A/S, Molecular Microbiology, 10-12 Bøge Allé, DK2970 Hørsholm, Denmark3

Received 20 March 2007/ Accepted 29 August 2007

Bile salts are natural detergents that facilitate the digestion and absorption of the hydrophobic components of the diet. However, their amphiphilic nature makes them very inhibitory for bacteria and strongly influences bacterial survival in the gastrointestinal tract. Adaptation to and tolerance of bile stress is therefore crucial for the persistence of bacteria in the human colonic niche. Bifidobacterium animalis subsp. lactis, a probiotic bacterium with documented health benefits, is applied largely in fermented dairy products. In this study, the effect of bile salts on proteomes of B. animalis subsp. lactis IPLA 4549 and its bile-resistant derivative B. animalis subsp. lactis 4549dOx was analyzed, leading to the identification of proteins which may represent the targets of bile salt response and adaptation in B. animalis subsp. lactis. The comparison of the wild-type and the bile-resistant strain responses allowed us to hypothesize about the resistance mechanisms acquired by the derivative resistant strain and about the bile salt response in B. animalis subsp. lactis. In addition, significant differences in the levels of metabolic end products of the bifid shunt and in the redox status of the cells were also detected, which correlate with some differences observed between the proteomes. These results indicate that adaptation and response to bile in B. animalis subsp. lactis involve several physiological mechanisms that are jointly dedicated to reduce the deleterious impact of bile on the cell's physiology.

* Corresponding author. Mailing address: Unité Flore Lactique et Environnement Carné (UR309), INRA, Domaine de Vilvert, F-78350 Jouy-en-Josas, France. Phone: 33 (0)1 34 65 22 89. Fax: 33 (0)1 34 65 21 05. E-mail: Marie-Christine.Champomier-Verges{at}jouy.inra.fr

{triangledown} Published ahead of print on 7 September 2007.

Applied and Environmental Microbiology, November 2007, p. 6757-6767, Vol. 73, No. 21
0099-2240/07/$08.00+0     doi:10.1128/AEM.00637-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gueimonde, M., Garrigues, C., van Sinderen, D., de los Reyes-Gavilan, C. G., Margolles, A. (2009). Bile-Inducible Efflux Transporter from Bifidobacterium longum NCC2705, Conferring Bile Resistance. Appl. Environ. Microbiol. 75: 3153-3160 [Abstract] [Full Text]  
  • Gagnaire, V., Jardin, J., Jan, G., Lortal, S. (2009). Invited review: Proteomics of milk and bacteria used in fermented dairy products: From qualitative to quantitative advances. J DAIRY SCI 92: 811-825 [Abstract] [Full Text]  
  • Ruas-Madiedo, P., Gueimonde, M., Arigoni, F., de los Reyes-Gavilan, C. G., Margolles, A. (2009). Bile Affects the Synthesis of Exopolysaccharides by Bifidobacterium animalis. Appl. Environ. Microbiol. 75: 1204-1207 [Abstract] [Full Text]  


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