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Applied and Environmental Microbiology, November 2007, p. 7041-7047, Vol. 73, No. 21
0099-2240/07/$08.00+0     doi:10.1128/AEM.00436-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Induction of Immune Responses in Mice after Intragastric Administration of Lactobacillus casei Producing Porcine Parvovirus VP2 Protein{triangledown}

Yigang Xu and Yijing Li*

Veterinary Department, Northeast Agricultural University, Harbin 150030, People's Republic of China

Received 25 June 2007/ Accepted 27 August 2007

Lactobacillus casei ATCC 393 was selected as an antigen delivery vehicle for mucosal immunization against porcine parvovirus (PPV) infection. A 64-kDa fragment of PPV major protective antigen VP2 protein was used as the parvovirus antigen model. A recombinant Lactobacillus expressing VP2 protein was constructed with plasmid pPG611.1, where expression and localization of the VP2 protein from recombinant Lc393-rPPV-VP2 was detected via sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blotting, and immunofluorescence. Both local mucosal and systemic immune responses against PPV were induced in BALB/c mice immunized orally with the recombinant Lactobacillus expressing VP2 protein. The induced antibodies demonstrated neutralizing effects on PPV infection. These data indicated that the use of recombinant lactobacilli could be a valuable strategy for future vaccine development of PPV.

* Corresponding author. Mailing address: Veterinary Department, Northeast Agricultural University, Harbin 150030, People's Republic of China. Phone: 86-451-55191410. Fax: 86-451-55103336. E-mail: yijingli{at}yahoo.com

{triangledown} Published ahead of print on 7 September 2007.

Applied and Environmental Microbiology, November 2007, p. 7041-7047, Vol. 73, No. 21
0099-2240/07/$08.00+0     doi:10.1128/AEM.00436-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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