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Applied and Environmental Microbiology, November 2007, p. 7110-7113, Vol. 73, No. 21
0099-2240/07/$08.00+0     doi:10.1128/AEM.01108-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Quantification of Cell Proliferation and Alpha-Toxin Gene Expression of Clostridium perfringens in the Development of Necrotic Enteritis in Broiler Chickens{triangledown}

Weiduo Si,1,{dagger} Joshua Gong,1* Yanming Han,2 Hai Yu,1 John Brennan,2 Huaijun Zhou,1,{ddagger} and Shu Chen3

Food Research Program, Agriculture & Agri-Food Canada, Guelph, Ontario, Canada N1G 5C9,1 Nutreco Canada Agresearch, Guelph, Ontario, Canada N1G 4T2,2 Laboratory Services Division, University of Guelph, Guelph, Ontario, Canada N1H 8JT3

Received 9 May 2007/ Accepted 26 August 2007

Cell proliferation and alpha-toxin gene expression of Clostridium perfringens in relation to the development of necrotic enteritis (NE) were investigated. Unlike bacitracin-treated chickens, non-bacitracin-treated birds exhibited typical NE symptoms and reduced growth performance. They also demonstrated increased C. perfringens proliferation and alpha-toxin gene expression that were positively correlated and progressed according to the regression model y = b0 + b1X – b2X2. The average C. perfringens count of 5 log10 CFU/g in the ileal digesta appears to be a threshold for developing NE with a lesion score of 2.

* Corresponding author. Mailing address: Food Research Program, Agriculture and Agri-Food Canada, 93 Stone Road West, Guelph, Ontario, Canada N1G 5C9. Phone: (519) 780-8027. Fax: (519) 829-2600. E-mail: gongj{at}agr.gc.ca

{triangledown} Published ahead of print on 7 September 2007.

{dagger} Present address: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada.

{ddagger} Present address: Department of Poultry Science, Texas A&M University, College Station, TX.

Applied and Environmental Microbiology, November 2007, p. 7110-7113, Vol. 73, No. 21
0099-2240/07/$08.00+0     doi:10.1128/AEM.01108-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lu, Y., Sarson, A. J., Gong, J., Zhou, H., Zhu, W., Kang, Z., Yu, H., Sharif, S., Han, Y. (2009). Expression Profiles of Genes in Toll-Like Receptor-Mediated Signaling of Broilers Infected with Clostridium perfringens. CVI 16: 1639-1647 [Abstract] [Full Text]  
  • Zhou, H., Gong, J., Brisbin, J., Yu, H., Sarson, A. J., Si, W., Sharif, S., Han, Y. (2009). Transcriptional profiling analysis of host response to Clostridium perfringens infection in broilers. Poult. Sci. 88: 1023-1032 [Abstract] [Full Text]  


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