This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ufnar, J. A.
Right arrow Articles by Ellender, R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ufnar, J. A.
Right arrow Articles by Ellender, R. D.
Agricola
Right arrow Articles by Ufnar, J. A.
Right arrow Articles by Ellender, R. D.

 Previous Article

Applied and Environmental Microbiology, November 2007, p. 7118-7121, Vol. 73, No. 21
0099-2240/07/$08.00+0     doi:10.1128/AEM.00911-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Methanobrevibacter ruminantium as an Indicator of Domesticated-Ruminant Fecal Pollution in Surface Waters{triangledown}

Jennifer A. Ufnar,1* Shiao Y. Wang,1 David F. Ufnar,2 and R. D. Ellender1

Department of Biological Sciences, The University of Southern Mississippi, Hattiesburg, Mississippi 39406,1 Department of Geography and Geology, The University of Southern Mississippi, Hattiesburg, Mississippi 394062

Received 23 April 2007/ Accepted 30 August 2007

A PCR-based assay (Mrnif) targeting the nifH gene of Methanobrevibacter ruminantium was developed to detect fecal pollution from domesticated ruminants in environmental water samples. The assay produced the expected amplification product only when the reaction mixture contained DNA extracted from M. ruminantium culture, bovine (80%), sheep (100%), and goat (75%) feces, and water samples from a bovine waste lagoon (100%) and a creek contaminated with bovine lagoon waste (100%). The assay appears to be specific and sensitive and can distinguish between domesticated- and nondomesticated-ruminant fecal pollution in environmental samples.

* Corresponding author. Present address: Center for Science Outreach, Vanderbilt University Medical Center, 806 Light Hall, Nashville, TN 37232-0670. Phone: (615) 322-7140. Fax: (615) 322-7132. E-mail: jennifer.a.ufnar{at}vanderbilt.edu

{triangledown} Published ahead of print on 7 September 2007.

Applied and Environmental Microbiology, November 2007, p. 7118-7121, Vol. 73, No. 21
0099-2240/07/$08.00+0     doi:10.1128/AEM.00911-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Steinberg, L. M., Regan, J. M. (2009). mcrA-Targeted Real-Time Quantitative PCR Method To Examine Methanogen Communities. Appl. Environ. Microbiol. 75: 4435-4442 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»