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Applied and Environmental Microbiology, November 2007, p. 7123-7127, Vol. 73, No. 22
0099-2240/07/$08.00+0     doi:10.1128/AEM.01289-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Real-Time Quantitative PCR Measurement of Ileal Lactobacillus salivarius Populations from Broiler Chickens To Determine the Influence of Farming Practices{triangledown} ,{dagger}

Sally A. Harrow,1 Velmurugu Ravindran,2 Ruth C. Butler,1 John W. Marshall,1 and Gerald W. Tannock3*

New Zealand Institute for Crop & Food Research Limited, Christchurch, New Zealand,1 Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand,2 Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand3

Received 10 June 2007/ Accepted 10 September 2007

A real-time quantitative PCR assay targeting a 16S-23S intergenic spacer region sequence was devised to measure the sizes of populations of Lactobacillus salivarius present in ileal digesta collected from broiler chickens. This species has been associated with deconjugation of bile salts in the small bowel and reduced broiler productivity. The assay was tested as a means of monitoring the sizes of L. salivarius populations from broilers fed diets with different compositions, maintained at different stocking densities, or given the antimicrobial drugs bacitracin and monensin in the feed. Stocking densities did not influence the numbers of L. salivarius cells in the ileum. A diet containing meat and bone meal reduced the size of the L. salivarius population relative to that of chickens given the control diet, as did administration of bacitracin and monensin in the feed. These changes in the target bacterial population were associated with improved broiler weight gain.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Otago, P.O. Box 56, Dunedin, New Zealand. Phone: 64 3 479 7713. Fax: 64 3 479 8540. E-mail: gerald.tannock{at}stonebow.otago.ac.nz

{triangledown} Published ahead of print on 21 September 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, November 2007, p. 7123-7127, Vol. 73, No. 22
0099-2240/07/$08.00+0     doi:10.1128/AEM.01289-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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