This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Newton, R. J.
Right arrow Articles by McMahon, K. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Newton, R. J.
Right arrow Articles by McMahon, K. D.
Agricola
Right arrow Articles by Newton, R. J.
Right arrow Articles by McMahon, K. D.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2007, p. 7169-7176, Vol. 73, No. 22
0099-2240/07/$08.00+0     doi:10.1128/AEM.00794-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Phylogenetic Ecology of the Freshwater Actinobacteria acI Lineage{triangledown} ,{dagger}

Ryan J. Newton,1 Stuart E. Jones,2 Matthew R. Helmus,3 and Katherine D. McMahon4*

Microbiology Doctoral Training Program, University of Wisconsin—Madison, Madison, Wisconsin 53706,1 Limnology and Marine Sciences, University of Wisconsin—Madison, Madison, Wisconsin 53706,2 Department of Zoology, University of Wisconsin—Madison, Madison, Wisconsin 53706,3 Department of Civil and Environmental Engineering, University of Wisconsin—Madison, Madison, Wisconsin 537064

Received 10 April 2007/ Accepted 27 August 2007

The acI lineage of freshwater Actinobacteria is a cosmopolitan and often numerically dominant member of lake bacterial communities. We conducted a survey of acI 16S rRNA genes and 16S-23S rRNA internal transcribed spacer regions from 18 Wisconsin lakes and used standard nonphylogenetic and phylogenetic statistical approaches to investigate the factors that determine acI community composition at the local scale (within lakes) and at the regional scale (across lakes). Phylogenetic reconstruction of 434 acI 16S rRNA genes revealed a well-defined and highly resolved phylogeny. Eleven previously unrecognized monophyletic clades, each with ≥97.9% within-clade 16S rRNA gene sequence identity, were identified. Clade community similarity positively correlated with lake environmental similarity but not with geographic distance, implying that the lakes represent a single biotic region containing environmental filters for communities that have similar compositions. Phylogenetically disparate clades within the acI lineage were most abundant at the regional scale, and local communities were comprised of more closely related clades. Lake pH was a strong predictor of the community composition, but only when lakes with a pH below 6 were included in the data set. In the remaining lakes (pH above 6) biogeographic patterns in the landscape were instead a predictor of the observed acI community structure. The nonrandom distribution of the newly defined acI clades suggests potential ecophysiological differences between the clades, with acI clades AI, BII, and BIII preferring acidic lakes and acI clades AII, AVI, and BI preferring more alkaline lakes.

* Corresponding author. Mailing address: Department of Civil and Environmental Engineering, University of Wisconsin—Madison, Madison, WI 53706. Phone: (608) 263-3137. Fax: (608) 262-5199. E-mail: tmcmahon{at}engr.wisc.edu

{triangledown} Published ahead of print on 7 September 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, November 2007, p. 7169-7176, Vol. 73, No. 22
0099-2240/07/$08.00+0     doi:10.1128/AEM.00794-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jezbera, J., Sharma, A. K., Brandt, U., Doolittle, W. F., Hahn, M. W. (2009). 'Candidatus Planktophila limnetica', an actinobacterium representing one of the most numerically important taxa in freshwater bacterioplankton. Int. J. Syst. Evol. Microbiol. 59: 2864-2869 [Abstract] [Full Text]  
  • Tarao, M., Jezbera, J., Hahn, M. W. (2009). Involvement of Cell Surface Structures in Size-Independent Grazing Resistance of Freshwater Actinobacteria. Appl. Environ. Microbiol. 75: 4720-4726 [Abstract] [Full Text]  
  • Kara, E., Shade, A. (2009). Temporal Dynamics of South End Tidal Creek (Sapelo Island, Georgia) Bacterial Communities. Appl. Environ. Microbiol. 75: 1058-1064 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»