This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mendes, R.
Right arrow Articles by Raaijmakers, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mendes, R.
Right arrow Articles by Raaijmakers, J. M.
Agricola
Right arrow Articles by Mendes, R.
Right arrow Articles by Raaijmakers, J. M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2007, p. 7259-7267, Vol. 73, No. 22
0099-2240/07/$08.00+0     doi:10.1128/AEM.01222-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Diversity of Cultivated Endophytic Bacteria from Sugarcane: Genetic and Biochemical Characterization of Burkholderia cepacia Complex Isolates{triangledown}

Rodrigo Mendes,1,2 Aline A. Pizzirani-Kleiner,1 Welington L. Araujo,1 and Jos M. Raaijmakers2*

Department of Genetics, University of São Paulo, 13400-970 Piracicaba SP, Brazil,1 Laboratory of Phytopathology, Wageningen University, 6709 PD Wageningen, The Netherlands2

Received 1 June 2007/ Accepted 20 September 2007

Bacteria were isolated from the rhizosphere and from inside the roots and stems of sugarcane plants grown in the field in Brazil. Endophytic bacteria were found in both the roots and the stems of sugarcane plants, with a significantly higher density in the roots. Many of the cultivated endophytic bacteria were shown to produce the plant growth hormone indoleacetic acid, and this trait was more frequently found among bacteria from the stem. 16S rRNA gene sequence analysis revealed that the selected isolates of the endophytic bacterial community of sugarcane belong to the genera of Burkholderia, Pantoea, Pseudomonas, and Microbacterium. Bacterial isolates belonging to the genus Burkholderia were the most predominant among the endophytic bacteria. Many of the Burkholderia isolates produced the antifungal metabolite pyrrolnitrin, and all were able to grow at 37°C. Phylogenetic analyses of the 16S rRNA gene and recA gene sequences indicated that the endophytic Burkholderia isolates from sugarcane are closely related to clinical isolates of the Burkholderia cepacia complex and clustered with B. cenocepacia (gv. III) isolates from cystic fibrosis patients. These results suggest that isolates of the B. cepacia complex are an integral part of the endophytic bacterial community of sugarcane in Brazil and reinforce the hypothesis that plant-associated environments may act as a niche for putative opportunistic human pathogenic bacteria.

* Corresponding author. Mailing address: Laboratory of Phytopathology, Section of Molecular Ecology, Wageningen University, Binnenhaven 5, 6709 PD Wageningen, The Netherlands. Phone: (31) 317 483 427. Fax: (31) 317 483 412. E-mail: jos.raaijmakers{at}wur.nl

{triangledown} Published ahead of print on 28 September 2007.

Applied and Environmental Microbiology, November 2007, p. 7259-7267, Vol. 73, No. 22
0099-2240/07/$08.00+0     doi:10.1128/AEM.01222-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»