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Applied and Environmental Microbiology, November 2007, p. 7477-7481, Vol. 73, No. 22
0099-2240/07/$08.00+0     doi:10.1128/AEM.01118-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Evaluation of Postharvest-Processed Oysters by Using PCR-Based Most-Probable-Number Enumeration of Vibrio vulnificus Bacteria{triangledown}

Anita C. Wright,* Victor Garrido, Georgia Debuex, Melissa Farrell-Evans, Archana A. Mudbidri, and W. Steven Otwell

University of Florida, Department of Food Science and Human Nutrition, P.O. Box 110370, Gainesville, Florida 32611

Received 18 May 2007/ Accepted 18 September 2007

Postharvest processing (PHP) is used to reduce levels of Vibrio vulnificus in oysters, but process validation is labor-intensive and expensive. Therefore, quantitative PCR was evaluated as a rapid confirmation method for most-probable-number enumeration (QPCR-MPN) of V. vulnificus bacteria in PHP oysters. QPCR-MPN showed excellent correlation (R2 = 0.97) with standard MPN and increased assay sensitivity and efficiency.

* Corresponding author. Mailing address: University of Florida, Department of Food Science and Human Nutrition, P.O. Box 110370, Gainesville, FL 32611. Phone: (352) 392-1991, ext. 311. Fax: (352) 392-9467. E-mail: acw{at}ufl.edu

{triangledown} Published ahead of print on 28 September 2007.

Applied and Environmental Microbiology, November 2007, p. 7477-7481, Vol. 73, No. 22
0099-2240/07/$08.00+0     doi:10.1128/AEM.01118-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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