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 Previous Article

Applied and Environmental Microbiology, November 2007, p. 7494-7500, Vol. 73, No. 22
0099-2240/07/$08.00+0     doi:10.1128/AEM.00738-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Comparison of Direct Genome Restriction Enzyme Analysis and Pulsed-Field Gel Electrophoresis for Typing of Vibrio vulnificus and Their Correspondence with Multilocus Sequence Typing Data{triangledown}

Narjol González-Escalona,1,2* Brooke Whitney,1 Lee-Ann Jaykus,1 and Angelo DePaola2

Department of Food Science, North Carolina State University, Raleigh, North Carolina,1 FDA Gulf Coast Seafood Laboratory, Dauphin Island, Alabama2

Received 2 April 2007/ Accepted 13 August 2007

We compared the potential of direct genome restriction enzyme analysis (DGREA) and pulsed-field gel electrophoresis (PFGE) for discriminating Vibrio vulnificus isolates from clinical (23) and environmental (17) sources. The genotypes generated by both methodologies were compared to previous multilocus sequence typing (MLST) data. DGREA established clearer relationships among V. vulnificus strains and was more consistent with MLST than with PFGE. DGREA is a very promising tool for epidemiological and ecological studies of V. vulnificus.

* Corresponding author. Mailing address: FDA Gulf Coast Seafood Laboratory, 1 Iberville Dr., Dauphin Island, AL 36528. Phone: (251) 690-3074. Fax: (251) 694-4477. E-mail: narjol{at}gmail.com

{triangledown} Published ahead of print on 24 August 2007.

Applied and Environmental Microbiology, November 2007, p. 7494-7500, Vol. 73, No. 22
0099-2240/07/$08.00+0     doi:10.1128/AEM.00738-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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