This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hibi, M.
Right arrow Articles by Mori, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hibi, M.
Right arrow Articles by Mori, H.
Agricola
Right arrow Articles by Hibi, M.
Right arrow Articles by Mori, H.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, December 2007, p. 7657-7663, Vol. 73, No. 23
0099-2240/07/$08.00+0     doi:10.1128/AEM.01754-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Improvement of NADPH-Dependent Bioconversion by Transcriptome-Based Molecular Breeding{triangledown} ,{dagger}

Makoto Hibi, Hiromi Yukitomo, Mikito Ito, and Hideo Mori*

Biofrontier Laboratories, Kyowa Hakko Kogyo Co. Ltd., 3-6-6 Asahimachi, Machida, Tokyo 194-8533, Japan

Received 28 July 2007/ Accepted 27 September 2007

Transcriptome data for a xylitol-producing recombinant Escherichia coli were obtained and used to tune up its productivity. Structural genes of NADPH-dependent D-xylose reductase and D-xylose permease were inserted into an Escherichia coli chromosome to construct a recombinant strain producing xylitol from D-xylose for use as a model system for NADPH-dependent bioconversion. Transcriptome analysis of xylitol-producing and nonproducing conditions for the recombinant revealed that xylitol production down-regulated 56 genes. These genes were then selected as candidate factors for suppression of the NADPH supply and were disrupted to validate their functions. Of the gene disruptants, that resulting from the deletion of yhbC showed the best bioconversion rate. Also, the deletion accelerated cell growth during log phase. The features of the mutant could be maintained in jar fermenter-scale production of xylitol. Thus, our novel molecular host strain breeding method using transcriptome analysis was fully effective and could be applied to improving various industrial strains.

* Corresponding author. Mailing address: Biofrontier Laboratories, Kyowa Hakko Kogyo Co. Ltd., 3-6-6 Asahimachi, Machida, Tokyo 194-8533, Japan. Phone: 81-42-726-2555. Fax: 81-42-726-8330. E-mail: hmori{at}kyowa.co.jp

{triangledown} Published ahead of print on 5 October 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, December 2007, p. 7657-7663, Vol. 73, No. 23
0099-2240/07/$08.00+0     doi:10.1128/AEM.01754-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»