This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cronin, M.
Right arrow Articles by van Sinderen, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cronin, M.
Right arrow Articles by van Sinderen, D.
Agricola
Right arrow Articles by Cronin, M.
Right arrow Articles by van Sinderen, D.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, December 2007, p. 7858-7866, Vol. 73, No. 24
0099-2240/07/$08.00+0     doi:10.1128/AEM.01630-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Molecular Dissection of a Bifidobacterial Replicon{triangledown} ,{dagger}

Michelle Cronin, Moritz Knobel, Mary O'Connell-Motherway, Gerald F. Fitzgerald, and Douwe van Sinderen*

Alimentary Pharmabiotic Centre and Department of Microbiology, University College Cork, Western Road, Cork, Ireland

Received 17 July 2007/ Accepted 30 September 2007

The 2.1-kb cryptic plasmid pCIBAO89 from Bifidobacterium asteroides harbors a 1.4-kb segment which is sufficient for its autonomous replication. The segment is divided into two parts, the presumed replication origin, ori89, and the rep gene encoding the putative 41-kDa Rep89 replication initiation protein. This minimal replication region of pCIBAO89 was functionally dissected by transcriptional analyses as well as by DNA-binding studies, and the information obtained was exploited to create a number of Escherichia coli-Bifidobacterium shuttle vectors capable of transforming various bifidobacteria with an efficiency of up to 106 transformants/µg DNA.

* Corresponding author. Mailing address: Department of Microbiology, National University of Ireland, Western Road, Cork, Ireland. Phone: 00 353 214091365. Fax: 00 353 214903101. E-mail: d.vansinderen{at}ucc.ie

{triangledown} Published ahead of print on 26 October 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, December 2007, p. 7858-7866, Vol. 73, No. 24
0099-2240/07/$08.00+0     doi:10.1128/AEM.01630-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Alvarez-Martin, P., Belen Florez, A., Margolles, A., del Solar, G., Mayo, B. (2008). Improved Cloning Vectors for Bifidobacteria, Based on the Bifidobacterium catenulatum pBC1 Replicon. Appl. Environ. Microbiol. 74: 4656-4665 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»