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Applied and Environmental Microbiology, December 2007, p. 7959-7966, Vol. 73, No. 24
0099-2240/07/$08.00+0     doi:10.1128/AEM.01657-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Epidemiology, Relative Invasive Ability, Molecular Characterization, and Competitive Performance of Campylobacter jejuni Strains in the Chicken Gut

Christopher Pope,¹ Janet Wilson,² Eduardo N. Taboada,³ Joanne MacKinnon,⁴ Cristiano A. Felipe Alves,¹ John H. E. Nash,³ Kris Rahn,⁴ and Gerald W. Tannock^1*

Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand,¹ Southern Community Laboratories, Dunedin, New Zealand,² Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada,³ Laboratory for Foodborne Zoonoses, Health Canada, Guelph, Ontario, Canada⁴

Received 19 July 2007/ Accepted 20 September 2007

One hundred forty-one Campylobacter jejuni isolates from humans with diarrhea and 100 isolates from retailed poultry meat were differentiated by flaA typing. The bacteria were isolated in a specific geographical area (Dunedin) in New Zealand over a common time period. Twenty nine flaA types were detected, one of which (flaA restriction fragment length polymorphism type 15 [flaA-15]) predominated among isolates from humans (30% of isolates). This strain was of low prevalence (5% of isolates) among poultry isolates. flaA-15 strains were five to six times more invasive of HEp2 cells in an in vitro assay than a flaA type (flaA-3) that was commonly encountered on poultry meat (23% of isolates) but was seldom associated with human illness (5%). Competitive-exclusion experiments with chickens, utilizing real-time quantitative PCR to measure the population sizes of specific strains representing flaA-15 (T1016) and flaA-3 (Pstau) in digesta, were carried out. These experiments showed that T1016 always outcompeted Pstau in the chicken intestine. Genomic comparisons of T1016 and Pstau were made using DNA microarrays representing the genome of C. jejuni NCTC 11168. These comparisons revealed differences between the strains in the gene content of the Cj1417c-to-Cj1442c region of the genome, which is associated with the formation of capsular polysaccharide. The strains differed in Penner type (T1016, O42; Pstau, O53). It was concluded that poultry meat was at least one source of human infection with C. jejuni, that some Campylobacter strains detected in poultry meat are of higher virulence for humans than others, and that bacterial attributes affecting strain virulence and commensal colonization ability may be linked.

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* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Otago, P.O. Box 56, Dunedin, New Zealand. Phone: 64 3 479 7713. Fax: 64 3 479 8540. E-mail: gerald.tannock{at}stonebow.otago.ac.nz

Published ahead of print on 5 October 2007.

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Applied and Environmental Microbiology, December 2007, p. 7959-7966, Vol. 73, No. 24
0099-2240/07/$08.00+0     doi:10.1128/AEM.01657-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

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