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Density Gradient Enrichment of Escherichia coli Conditional msbA Mutants

Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803

Received 31 August 2007/ Accepted 18 October 2007

Insight into the mechanism of lipid transport to the outer membrane of gram-negative bacteria has been hampered by the lack of an effective genetic screen for defective mutants. This work demonstrates an enrichment of conditional mutants defective in lipopolysaccharide export by Ludox density gradient centrifugation and selection for detergent resistance. New temperature-sensitive mutants with lipid export defects were isolated with single missense mutations in msbA. The results demonstrate the power of this approach for the study of lipid export in Escherichia coli.

Published ahead of print on 26 October 2007.

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