AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
AEM.01992-07v1
73/24/7992    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Doerrler, W. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Doerrler, W. T.
Agricola
Right arrow Articles by Doerrler, W. T.
Applied and Environmental Microbiology, December 2007, p. 7992-7996, Vol. 73, No. 24
0099-2240/07/$08.00+0     doi:10.1128/AEM.01992-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Density Gradient Enrichment of Escherichia coli Conditional msbA Mutants{triangledown}

William T. Doerrler*

Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803

Received 31 August 2007/ Accepted 18 October 2007

Insight into the mechanism of lipid transport to the outer membrane of gram-negative bacteria has been hampered by the lack of an effective genetic screen for defective mutants. This work demonstrates an enrichment of conditional mutants defective in lipopolysaccharide export by Ludox density gradient centrifugation and selection for detergent resistance. New temperature-sensitive mutants with lipid export defects were isolated with single missense mutations in msbA. The results demonstrate the power of this approach for the study of lipid export in Escherichia coli.


* Corresponding author. Mailing address: Department of Biological Sciences, Louisiana State University, 202 Life Sciences Building, Baton Rouge, LA 70803. Phone: (225) 578-7904. Fax: (225) 578-2597. E-mail: wdoerr{at}lsu.edu

{triangledown} Published ahead of print on 26 October 2007.


Applied and Environmental Microbiology, December 2007, p. 7992-7996, Vol. 73, No. 24
0099-2240/07/$08.00+0     doi:10.1128/AEM.01992-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.




This article has been cited by other articles:




Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2007 by the American Society for Microbiology. All rights reserved.