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Applied and Environmental Microbiology, December 2007, p. 7997-8000, Vol. 73, No. 24
0099-2240/07/$08.00+0     doi:10.1128/AEM.00956-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Reconstitution of Glyphosate Resistance from a Split 5-Enolpyruvyl Shikimate-3-Phosphate Synthase Gene in Escherichia coli and Transgenic Tobacco{triangledown} ,{dagger}

Bao-Qing Dun,1,2,{ddagger} Xu-Jing Wang,1,{ddagger} Wei Lu,1 Zhong-Lin Zhao,1,3 Song-Na Hou,1 Bao-Ming Zhang,2 Gui-Ying Li,2 Thomas C. Evans Jr.,4 Ming-Qun Xu,4 and Min Lin1*

Biotechnology Research Institute, Chinese Academy of Agriculture Sciences, Beijing 100081, China,1 National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agriculture Sciences, Beijing 100081, China,2 College of Biological Sciences, China Agricultural University, Beijing 100094, China,3 New England Biolabs, Inc., Ipswich, Massachusetts 019384

Received 28 April 2007/ Accepted 10 October 2007

A highly N-phosphonomethylglycine (glyphosate)-resistant Pseudomonas fluorescens G2 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) was mapped to identify potential split sites using a transposon-based linker-scanning procedure. Intein-mediated protein complementation was used to reconstitute glyphosate resistance from the genetically divided G2 EPSPS gene in Escherichia coli strain ER2799 and transgenic tobacco.

* Corresponding author. Mailing address: Biotechnology Research Institute, Chinese Academy of Agriculture Sciences, Beijing 100081, China. Phone: 86-10-62139578. Fax: 86-10-62136981. E-mail: linmin57{at}vip.163.com

{triangledown} Published ahead of print on 19 October 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

{ddagger} B.-Q.D. and X.-J.W. contributed to the study equally.

Applied and Environmental Microbiology, December 2007, p. 7997-8000, Vol. 73, No. 24
0099-2240/07/$08.00+0     doi:10.1128/AEM.00956-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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