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Applied and Environmental Microbiology, December 2007, p. 8028-8031, Vol. 73, No. 24
0099-2240/07/$08.00+0     doi:10.1128/AEM.01198-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Enumeration of Viable Listeria monocytogenes Cells by Real-Time PCR with Propidium Monoazide and Ethidium Monoazide in the Presence of Dead Cells{triangledown} ,{dagger}

Y. Pan and F. Breidt Jr.*

U.S. Department of Agriculture, Agricultural Research Service, and North Carolina Agricultural Research Service, Department of Microbiology, North Carolina State University, Raleigh, North Carolina 27695-7624

Received 29 May 2007/ Accepted 7 October 2007

Propidium monoazide (PMA) and ethidium monoazide were used for enumeration of viable Listeria monocytogenes cells in the presence of dead cells. PMA had no antimicrobial effect on L. monocytogenes. Viable cell counts were linearly related to real-time PCR threshold cycle values for PMA-treated cells from planktonic and biofilm sources over a 4-log range.

* Corresponding author. Mailing address: U.S. Department of Agriculture, Agricultural Research Service, and North Carolina Agricultural Research Service, Department of Microbiology, North Carolina State University, Raleigh, NC 27695-7624. Phone: (919) 513-0186. Fax: (919) 513-0180. E-mail: Fred.Breidt{at}ars.usda.gov

{triangledown} Published ahead of print on 12 October 2007.

{dagger} Paper no. FSR07-09 of the Journal Series of the Department of Food Science, North Carolina State University, Raleigh.

Applied and Environmental Microbiology, December 2007, p. 8028-8031, Vol. 73, No. 24
0099-2240/07/$08.00+0     doi:10.1128/AEM.01198-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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