Previous Article | Next Article 
Applied and Environmental Microbiology, December 2007, p. 8028-8031, Vol. 73, No. 24
0099-2240/07/$08.00+0 doi:10.1128/AEM.01198-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Enumeration of Viable Listeria monocytogenes Cells by Real-Time PCR with Propidium Monoazide and Ethidium Monoazide in the Presence of Dead Cells
,
Y. Pan and
F. Breidt Jr.*
U.S. Department of Agriculture, Agricultural Research Service, and North Carolina Agricultural Research Service, Department of Microbiology, North Carolina State University, Raleigh, North Carolina 27695-7624
Received 29 May 2007/
Accepted 7 October 2007
Propidium monoazide (PMA) and ethidium monoazide were used for enumeration of viable Listeria monocytogenes cells in the presence of dead cells. PMA had no antimicrobial effect on L. monocytogenes. Viable cell counts were linearly related to real-time PCR threshold cycle values for PMA-treated cells from planktonic and biofilm sources over a 4-log range.
* Corresponding author. Mailing address: U.S. Department of Agriculture, Agricultural Research Service, and North Carolina Agricultural Research Service, Department of Microbiology, North Carolina State University, Raleigh, NC 27695-7624. Phone: (919) 513-0186. Fax: (919) 513-0180. E-mail:
Fred.Breidt{at}ars.usda.gov
Published ahead of print on 12 October 2007.
Paper no. FSR07-09 of the Journal Series of the Department of Food Science, North Carolina State University, Raleigh.
Applied and Environmental Microbiology, December 2007, p. 8028-8031, Vol. 73, No. 24
0099-2240/07/$08.00+0 doi:10.1128/AEM.01198-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.
This article has been cited by other articles:
-
Brescia, C. C., Griffin, S. M., Ware, M. W., Varughese, E. A., Egorov, A. I., Villegas, E. N.
(2009). Cryptosporidium Propidium Monoazide-PCR, a Molecular Biology-Based Technique for Genotyping of Viable Cryptosporidium Oocysts. Appl. Environ. Microbiol.
75: 6856-6863
[Abstract]
[Full Text]
-
Pan, Y., Breidt, F. Jr., Kathariou, S.
(2009). Competition of Listeria monocytogenes Serotype 1/2a and 4b Strains in Mixed-Culture Biofilms. Appl. Environ. Microbiol.
75: 5846-5852
[Abstract]
[Full Text]
-
Bae, S., Wuertz, S.
(2009). Discrimination of Viable and Dead Fecal Bacteroidales Bacteria by Quantitative PCR with Propidium Monoazide. Appl. Environ. Microbiol.
75: 2940-2944
[Abstract]
[Full Text]
-
Reichert-Schwillinsky, F., Pin, C., Dzieciol, M., Wagner, M., Hein, I.
(2009). Stress- and Growth Rate-Related Differences between Plate Count and Real-Time PCR Data during Growth of Listeria monocytogenes. Appl. Environ. Microbiol.
75: 2132-2138
[Abstract]
[Full Text]
-
Chang, B., Sugiyama, K., Taguri, T., Amemura-Maekawa, J., Kura, F., Watanabe, H.
(2009). Specific Detection of Viable Legionella Cells by Combined Use of Photoactivated Ethidium Monoazide and PCR/Real-Time PCR. Appl. Environ. Microbiol.
75: 147-153
[Abstract]
[Full Text]