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Applied and Environmental Microbiology, February 2007, p. 1019-1024, Vol. 73, No. 3
0099-2240/07/$08.00+0     doi:10.1128/AEM.01670-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Luminescent Whole-Cell Cyanobacterial Bioreporter for Measuring Fe Availability in Diverse Marine Environments{triangledown}

Ramakrishna Boyanapalli,1,{dagger} George S. Bullerjahn,1* Christa Pohl,2 Peter L. Croot,3 Philip W. Boyd,4 and R. Michael L. McKay1

Department of Biological Sciences, Bowling Green State University, Bowling Green, Ohio 43403,1 Sektion Meereschemie, Leibniz Institut für Ostseeforschung Warnemünde, Warnemünde, Germany,2 Marine Biogeochemie, Leibniz Institut für Meereswissenschaften an der Universität Kiel (IfM-GEOMAR), Kiel, Germany,3 National Institute of Water and Atmospheric Research, Centre for Chemical and Physical Oceanography, Department of Chemistry, University of Otago, Dunedin, New Zealand4

Received 18 July 2006/ Accepted 30 November 2006

A Synechococcus sp. strain PCC 7002 Fe bioreporter was constructed containing the isiAB promoter fused to the Vibrio harveyi luxAB genes. Bioreporter luminescence was characterized with respect to the free ferric ion concentration in trace metal-buffered synthetic medium. The applicability of the Fe bioreporter to assess Fe availability in the natural environment was tested by using samples collected from the Baltic Sea and from the high-nutrient, low-chlorophyll subarctic Pacific Ocean. Parallel assessment of dissolved Fe and bioreporter response confirmed that direct chemical measurements of dissolved Fe should not be considered alone when assessing Fe availability to phytoplankton.

* Corresponding author. Mailing address: Department of Biological Sciences, Bowling Green State University, Bowling Green, OH 43403. Phone: (419) 372-8527. Fax: (419) 372-2024. E-mail: bullerj{at}bgsu.edu.

{triangledown} Published ahead of print on 8 December 2006.

{dagger} Present address: University of Colorado at Boulder, Boulder, CO 80309-0215.

Applied and Environmental Microbiology, February 2007, p. 1019-1024, Vol. 73, No. 3
0099-2240/07/$08.00+0     doi:10.1128/AEM.01670-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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