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Applied and Environmental Microbiology, February 2007, p. 1029-1032, Vol. 73, No. 3
0099-2240/07/$08.00+0     doi:10.1128/AEM.01780-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Prevalence of ColE1-Like Plasmids and Colicin K Production among Uropathogenic Escherichia coli Strains and Quantification of Inhibitory Activity of Colicin K{triangledown}

Matija Rijavec,1 Maruska Budic,1 Peter Mrak,1,{dagger} Manica Müller-Premru,2 Zdravko Podlesek,1 and Darja Zgur-Bertok1*

Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia,1 Institute for Microbiology and Immunology, Medical Faculty, University of Ljubljana, Ljubljana, Slovenia2

Received 27 July 2006/ Accepted 12 November 2006

Colicin K exhibited pronounced inhibitory activity against uropathogenic Escherichia coli (UPEC) strains. Low prevalence of colicin K production and a relatively high prevalence of ColE1-like plasmids were determined among 215 UPEC strains from Slovenia. Sequencing of the colicin K-encoding pColK-K235 revealed a mosaic structure and the presence of the insertion sequence IS2.

* Corresponding author. Mailing address: Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, Ljubljana, Slovenia. Phone: 368 1 423 3388. Fax: 386 1 257 3390. E-mail: darja.zgur.bertok{at}bf.uni-lj.si.

{triangledown} Published ahead of print on 22 November 2006.

{dagger} Present address: Lek Pharmaceuticals d.d., Menges, Slovenia.

Applied and Environmental Microbiology, February 2007, p. 1029-1032, Vol. 73, No. 3
0099-2240/07/$08.00+0     doi:10.1128/AEM.01780-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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