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Applied and Environmental Microbiology, February 2007, p. 706-710, Vol. 73, No. 3
0099-2240/07/$08.00+0     doi:10.1128/AEM.01082-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Evaluation of a Wipe Surface Sample Method for Collection of Bacillus Spores from Nonporous Surfaces{triangledown}

Gary S. Brown,1* Rita G. Betty,2 John E. Brockmann,3 Daniel A. Lucero,3 Caroline A. Souza,1 Kathryn S. Walsh,1 Raymond M. Boucher,4 Mathew Tezak,5 Mollye C. Wilson,5 and Todd Rudolph6

Sandia National Laboratories, P.O. Box 5800, MS0734, Albuquerque, New Mexico 87185,1 Sandia National Laboratories, P.O. Box 5800, MS0115, Albuquerque, New Mexico 87185,2 Sandia National Laboratories, P.O. Box 5800, MS0836, Albuquerque, New Mexico 87185,3 Orion International Technologies, P.O. Box 5800, MS0734, Albuquerque, New Mexico 87185,4 American Staff Augmentation Providers, P.O. Box 5800, MS0734, Albuquerque, New Mexico 87185,5 Tactical Staffing Resources, P.O. Box 5800, MS0836, Albuquerque, New Mexico 871856

Received 10 May 2006/ Accepted 12 November 2006

Polyester-rayon blend wipes were evaluated for efficiency of extraction and recovery of powdered Bacillus atrophaeus spores from stainless steel and painted wallboard surfaces. Method limits of detection were also estimated for both surfaces. The observed mean efficiency of polyester-rayon blend wipe recovery from stainless steel was 0.35 with a standard deviation of ±0.12, and for painted wallboard it was 0.29 with a standard deviation of ±0.15. Evaluation of a sonication extraction method for the polyester-rayon blend wipes produced a mean extraction efficiency of 0.93 with a standard deviation of ±0.09. Wipe recovery quantitative limits of detection were estimated at 90 CFU per unit of stainless steel sample area and 105 CFU per unit of painted wallboard sample area. The method recovery efficiency and limits of detection established in this work provide useful guidance for the planning of incident response environmental sampling following the release of a biological agent such as Bacillus anthracis.

* Corresponding author. Mailing address: Sandia National Laboratories, P.O. Box 5800, MS0734, Albuquerque, NM 87185. Phone: (505) 845-8312. Fax: (505) 844-7786. E-mail: gbrown{at}sandia.gov.

{triangledown} Published ahead of print on 22 November 2006.

Applied and Environmental Microbiology, February 2007, p. 706-710, Vol. 73, No. 3
0099-2240/07/$08.00+0     doi:10.1128/AEM.01082-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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