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Applied and Environmental Microbiology, February 2007, p. 855-860, Vol. 73, No. 3
0099-2240/07/$08.00+0     doi:10.1128/AEM.02001-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Inhibitory Impact of Bifidobacteria on the Transfer of ß-Lactam Resistance among Enterobacteriaceae in the Gnotobiotic Mouse Digestive Tract{triangledown}

C. Moubareck,1 M. Lecso,1 E. Pinloche,1 M. J. Butel,1 and F. Doucet-Populaire1,2*

Laboratoire de Microbiologie, EA 4065, UFR des Sciences Pharmaceutiques et Biologiques, Université René Descartes-Paris 5, 75270 Paris Cedex 06, France,1 Centre Hospitalier de Versailles, Le Chesnay, France2

Received 23 August 2006/ Accepted 15 November 2006

While looking for new means to limit the dissemination of antibiotic resistance, we evaluated the role of potentially probiotic bifidobacteria on the transfer of resistance genes between enterobacteria. Transfers of bla genes encoding extended-spectrum ß-lactamases (SHV-5 and CTX-M-15) were studied in the absence or presence of bifidobacteria. In vitro, transfer frequencies of these bla genes decreased significantly in the presence of three of five tested strains, i.e., Bifidobacterium longum CUETM-89-215, Bifidobacterium bifidum CIP-56.7T, and Bifidobacterium pseudocatenulatum CIP-104168T. Four transfer experiments were conducted in the digestive tract of gnotobiotic mice, the first three observing the effect of B. longum CUETM-89-215, B. bifidum CIP-56.7T, and B. pseudocatenulatum CIP-104168T on blaSHV-5 transfer and the fourth experiment studying the effect of B. bifidum CIP-56.7T on blaCTX-M-15 transfer. These experiments revealed significant decreases in the transconjugant levels (up to 3 logs) in mice having received B. bifidum CIP-56.7T or B. pseudocatenulatum CIP-104168T compared to control mice. Bifidobacteria appear to have an inhibitory impact on the transfer of antibiotic resistance genes. The inhibitory effect is associated to specific bifidobacterial strains and may be related to the production of thermostable metabolites by these strains.

* Corresponding author. Mailing address: Faculté des Sciences Pharmaceutiques et Biologiques, Laboratoire de Microbiologie, 4 Avenue de l'Observatoire, 75270 Paris Cedex 06, France. Phone: (33) 1 53 73 99 13. Fax: (33) 1 53 73 99 23. E-mail: florence.doucet-populaire{at}univ-paris5.fr.

{triangledown} Published ahead of print on 22 November 2006.

Applied and Environmental Microbiology, February 2007, p. 855-860, Vol. 73, No. 3
0099-2240/07/$08.00+0     doi:10.1128/AEM.02001-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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