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Applied and Environmental Microbiology, February 2007, p. 861-868, Vol. 73, No. 3
0099-2240/07/$08.00+0     doi:10.1128/AEM.01818-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Role of Cytochrome bd Oxidase from Corynebacterium glutamicum in Growth and Lysine Production{triangledown}

Armin Kabus, Axel Niebisch, and Michael Bott*

Institut für Biotechnologie 1, Forschungszentrum Jülich, D-52425 Jülich, Germany

Received 2 August 2006/ Accepted 19 November 2006

Corynebacterium glutamicum possesses two terminal oxidases, cytochrome aa3 and cytochrome bd. Cytochrome aa3 forms a supercomplex with the cytochrome bc1 complex, which contains an unusual diheme cytochrome c1. Both the bc1-aa3 supercomplex and cytochrome bd transfer reducing equivalents from menaquinol to oxygen; however, they differ in their proton translocation efficiency by a factor of three. Here, we analyzed the role of cytochrome bd for growth and lysine production. When cultivated in glucose minimal medium, a cydAB deletion mutant of C. glutamicum ATCC 13032 grew like the wild type in the exponential phase, but growth thereafter was inhibited, leading to a biomass formation 40% less than that of the wild type. Constitutive overproduction of functional cytochrome bd oxidase in ATCC 13032 led to a reduction of the growth rate by ~45% and of the maximal biomass by ~35%, presumably as a consequence of increased electron flow through the inefficient cytochrome bd oxidase. In the L-lysine-producing C. glutamicum strain MH20-22B, deletion of the cydAB genes had only minor effects on growth rate and biomass formation, but lysine production was increased by ~12%. Thus, the respiratory chain was shown to be a target for improving amino acid production by C. glutamicum.

* Corresponding author. Mailing address: Institut für Biotechnologie 1, Forschungszentrum Jülich, D-52425 Jülich, Germany. Phone: 49 2461 615515. Fax: 49 2461 612710. E-mail: m.bott{at}fz-juelich.de.

{triangledown} Published ahead of print on 1 December 2006.

Applied and Environmental Microbiology, February 2007, p. 861-868, Vol. 73, No. 3
0099-2240/07/$08.00+0     doi:10.1128/AEM.01818-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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