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Applied and Environmental Microbiology, February 2007, p. 1049-1053, Vol. 73, No. 4
0099-2240/07/$08.00+0     doi:10.1128/AEM.01158-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Separation of Marine Bacteria according to Buoyant Density by Use of the Density-Dependent Cell Sorting Method{triangledown}

Katsuyuki Inoue,1* Masahiko Nishimura,1 Binaya B. Nayak,2 and Kazuhiro Kogure1

Ocean Research Institute, The University of Tokyo, Nakano, Tokyo 164-8639, Japan,1 Central Institute of Fisheries Education, Seven Bungalows, Versova, Mumbai 400 061, India2

Received 19 May 2006/ Accepted 1 December 2006

The purpose of this study was to test whether some phylogenetic groups of natural marine bacteria have unique buoyant densities that allow them to be separated by the density-dependent cell sorting (DDCS) method. We first concentrated a natural bacterial assemblage to collect sufficient numbers of cells. They were separated into three fractions by DDCS, and the community structure in each was clarified by fluorescence in situ hybridization. The cells of Archaea tended to appear in the high-density fraction, whereas those of Cytophaga-Flavobacterium-Bacteroides were in the low-density fraction. We also calculated the sedimentation velocities of three typical marine bacteria (low density, middle density, and high density) using their buoyant density. The sedimentation velocities were approximately 10, 20, and 30 µm h–1; these velocities have ecological implications when the heterogeneity of bacteria is considered at a microscale. To our knowledge, this is the first report on the buoyant density of natural marine bacteria.

* Corresponding author. Mailing address: Ocean Research Institute, The University of Tokyo, 1-15-1 Minamidai, Nakano, Tokyo 164-8639, Japan. Phone: 81 3 5351 6834. Fax: 81 3 5351 6482. E-mail: kix{at}ori.u-tokyo.ac.jp.

{triangledown} Published ahead of print on 8 December 2006.

Applied and Environmental Microbiology, February 2007, p. 1049-1053, Vol. 73, No. 4
0099-2240/07/$08.00+0     doi:10.1128/AEM.01158-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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