AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
AEM.02265-06v1
73/4/1107    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hyink, O.
Right arrow Articles by Tagg, J. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hyink, O.
Right arrow Articles by Tagg, J. R.
Agricola
Right arrow Articles by Hyink, O.
Right arrow Articles by Tagg, J. R.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, February 2007, p. 1107-1113, Vol. 73, No. 4
0099-2240/07/$08.00+0     doi:10.1128/AEM.02265-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Salivaricin A2 and the Novel Lantibiotic Salivaricin B Are Encoded at Adjacent Loci on a 190-Kilobase Transmissible Megaplasmid in the Oral Probiotic Strain Streptococcus salivarius K12{triangledown}

Otto Hyink,1 Philip A. Wescombe,2 Mathew Upton,1,3 Nancy Ragland,1 Jeremy P. Burton,1,2 and John R. Tagg1*

Department of Microbiology and Immunology, University of Otago, P.O. Box 56, Dunedin, New Zealand,1 BLIS Technologies Ltd., Centre for Innovation, P.O. Box 56, Dunedin, New Zealand,2 Manchester Medical Microbiology Partnership, University of Manchester School of Medicine, Manchester M13 9WL, United Kingdom3

Received 26 September 2006/ Accepted 17 December 2006

The commercial probiotic Streptococcus salivarius strain K12 is the prototype of those S. salivarius strains that are the most strongly inhibitory in a standardized test of streptococcal bacteriocin production and has been shown to produce the 2,368-Da salivaricin A2 (SalA2) and the 2,740-Da salivaricin B (SboB) lantibiotics. The previously uncharacterized SboB belongs to the type AII class of lantibiotic bacteriocins and is encoded by an eight-gene cluster. The genetic loci encoding SalA2 and SboB in strain K12 have been fully characterized and are localized to nearly adjacent sites on pSsal-K12, a 190-kb megaplasmid. Of 61 strongly inhibitory strains of S. salivarius, 19 (31%) were positive for the sboB structural gene. All but one (strain NR) of these 19 strains were also positive for salA2, and in each of these cases of double positivity, the two loci were separated by fewer than 10 kb. This is the first report of a single streptococcus strain producing two distinct lantibiotics.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Otago, P.O. Box 56, Dunedin, New Zealand. Phone: 643 479 7714. Fax: 643 479 8540. E-mail: john.tagg{at}stonebow.otago.ac.nz.

{triangledown} Published ahead of print on 28 December 2006.

Applied and Environmental Microbiology, February 2007, p. 1107-1113, Vol. 73, No. 4
0099-2240/07/$08.00+0     doi:10.1128/AEM.02265-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2007 by the American Society for Microbiology. All rights reserved.