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Applied and Environmental Microbiology, February 2007, p. 1308-1319, Vol. 73, No. 4
0099-2240/07/$08.00+0     doi:10.1128/AEM.01867-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Altered Metabolic Flux due to Deletion of odhA causes L-Glutamate Overproduction in Corynebacterium glutamicum{triangledown}

Yoko Asakura,1* Eiichiro Kimura,1 Yoshihiro Usuda,1 Yoshio Kawahara,1 Kazuhiko Matsui,1 Tsuyoshi Osumi,1 and Tsuyoshi Nakamatsu2

Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki, Kanagawa 210-8681,1 Department of Environmental Material Science, Tokyo Denki University, 2-2 Kandanishiki-cho, Chiyoda-ku, Tokyo 101-8457, Japan2

Received 7 August 2006/ Accepted 28 November 2006

L-Glutamate overproduction in Corynebacterium glutamicum, a biotin auxotroph, is induced by biotin limitation or by treatment with certain fatty acid ester surfactants or with penicillin. We have analyzed the relationship between the inductions, 2-oxoglutarate dehydrogenase complex (ODHC) activity, and L-glutamate production. Here we show that a strain deleted for odhA and completely lacking ODHC activity produces L-glutamate as efficiently as the induced wild type (27.8 mmol/g [dry weight] of cells for the ohdA deletion strain compared with only 1.0 mmol/g [dry weight] of cells for the uninduced wild type). This level of production is achieved without any induction or alteration in the fatty acid composition of the cells, showing that L-glutamate overproduction can be caused by the change in metabolic flux alone. Interestingly, the L-glutamate productivity of the odhA-deleted strain is increased about 10% by each of the L-glutamate-producing inductions, showing that the change in metabolic flux resulting from the odhA deletion and the inductions have additive effects on L-glutamate overproduction. Tween 40 was indicated to induce drastic metabolic change leading to L-glutamate overproduction in the odhA-deleted strain. Furthermore, optimizing the metabolic flux from 2-oxoglutarate to L-glutamate by tuning glutamate dehydrogenase activity increased the L-glutamate production of the odhA-deleted strain.

* Corresponding author. Mailing address: Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki, Kanagawa 210-8681, Japan. Phone: 81 (44) 244-7138. Fax: 81 (44) 244-7158. E-mail: youko_kuwabara{at}ajinomoto.com.

{triangledown} Published ahead of print on 8 December 2006.

Applied and Environmental Microbiology, February 2007, p. 1308-1319, Vol. 73, No. 4
0099-2240/07/$08.00+0     doi:10.1128/AEM.01867-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nakamura, J., Hirano, S., Ito, H., Wachi, M. (2007). Mutations of the Corynebacterium glutamicum NCgl1221 Gene, Encoding a Mechanosensitive Channel Homolog, Induce L-Glutamic Acid Production. Appl. Environ. Microbiol. 73: 4491-4498 [Abstract] [Full Text]  


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