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Applied and Environmental Microbiology, February 2007, p. 1375-1379, Vol. 73, No. 4
0099-2240/07/$08.00+0     doi:10.1128/AEM.01873-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Gateway-Based Destination Vectors for Functional Analyses of Bacterial ORFeomes: Application to the Min System in Brucella abortus{triangledown} ,{dagger}

Régis Hallez,{ddagger} Jean-Jacques Letesson, Jean Vandenhaute, and Xavier De Bolle*

Unité de Recherche en Biologie Moléculaire, University of Namur, 61 rue de Bruxelles, B-5000 Namur, Belgium

Received 7 August 2006/ Accepted 4 December 2006

Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscillating localization of MinD in Brucella abortus.

* Corresponding author. Mailing address: Unité de Recherche en Biologie Moléculaire, University of Namur, 61 rue de Bruxelles, B-5000 Namur, Belgium. Phone: 32 81 72 44 38. Fax: 32 81 72 42 97. E-mail: xavier.debolle{at}fundp.ac.be.

{triangledown} Published ahead of print on 15 December 2006.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

{ddagger} Present address: Laboratoire de Génétique des Procaryotes, Institut de Biologie et de Médecine Moléculaires, Université Libre de Bruxelles, 12 Rue des Professeurs Jeener et Brachet, B-6041 Gosselies, Belgium.

Applied and Environmental Microbiology, February 2007, p. 1375-1379, Vol. 73, No. 4
0099-2240/07/$08.00+0     doi:10.1128/AEM.01873-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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