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Applied and Environmental Microbiology, February 2007, p. 1383-1387, Vol. 73, No. 4
0099-2240/07/$08.00+0     doi:10.1128/AEM.02007-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Polyphosphate Accumulation by Pseudomonas putida CA-3 and Other Medium-Chain-Length Polyhydroxyalkanoate-Accumulating Bacteria under Aerobic Growth Conditions{triangledown}

Karen M. Tobin,1 John W. McGrath,2 Alan Mullan,2 John P. Quinn,2 and Kevin E. O'Connor1*

School of Biomolecular and Biomedical Sciences, College of Life Sciences, and Centre for Synthesis and Chemical Biology, the Conway Institute for Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland,1 School of Biology and Biochemistry and QUESTOR Centre, the Queen's University of Belfast, Medical Biology Centre, 97 Lisburn Rd., Belfast BT9 7BL, Ireland2

Received 23 August 2006/ Accepted 1 December 2006

Pseudomonas putida CA-3 accumulates polyphosphate (polyP) and medium-chain-length polyhydroxyalkanoate (mclPHA) concurrently under nitrogen limitation. Five other mclPHA-accumulating Pseudomonas strains are capable of simultaneous polyP and mclPHA biosynthesis. It appears that polyP is not the rate-limiting step for mclPHA accumulation in these Pseudomonas strains.


* Corresponding author. Mailing address: School of Biomolecular and Biomedical Sciences, College of Life Sciences, the Conway Institute for Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland. Phone: (353) 1 716 1307. Fax: (353) 1 716 1183. E-mail: kevin.oconnor{at}ucd.ie.

{triangledown} Published ahead of print on 8 December 2006.


Applied and Environmental Microbiology, February 2007, p. 1383-1387, Vol. 73, No. 4
0099-2240/07/$08.00+0     doi:10.1128/AEM.02007-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.