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Applied and Environmental Microbiology, February 2007, p. 1388-1392, Vol. 73, No. 4
0099-2240/07/$08.00+0     doi:10.1128/AEM.01921-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Metaproteomics Approach To Study the Functionality of the Microbiota in the Human Infant Gastrointestinal Tract{triangledown}

Eline S. Klaassens,* Willem M. de Vos, and Elaine E. Vaughan

Laboratory of Microbiology, Wageningen University, 6703 CT Wageningen, The Netherlands

Received 11 August 2006/ Accepted 3 December 2006

A metaproteomics approach comprising two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization-time of flight (mass spectrometry) was applied to the largely uncultured infant fecal microbiota for the first time. The fecal microbial metaproteome profiles changed over time, and one protein spot contained a peptide sequence that showed high similarity to those of bifidobacterial transaldolases.

* Corresponding author. Mailing address: Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands. Phone: 31 317 483486. Fax: 31 317 483829. E-mail: Eline.klaassens{at}wur.nl.

{triangledown} Published ahead of print on 8 December 2006.

Applied and Environmental Microbiology, February 2007, p. 1388-1392, Vol. 73, No. 4
0099-2240/07/$08.00+0     doi:10.1128/AEM.01921-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Klaassens, E. S., Boesten, R. J., Haarman, M., Knol, J., Schuren, F. H., Vaughan, E. E., de Vos, W. M. (2009). Mixed-Species Genomic Microarray Analysis of Fecal Samples Reveals Differential Transcriptional Responses of Bifidobacteria in Breast- and Formula-Fed Infants. Appl. Environ. Microbiol. 75: 2668-2676 [Abstract] [Full Text]  
  • Keller, M., Hettich, R. (2009). Environmental Proteomics: a Paradigm Shift in Characterizing Microbial Activities at the Molecular Level. Microbiol. Mol. Biol. Rev. 73: 62-70 [Abstract] [Full Text]  
  • Zoetendal, E G, Rajilic-Stojanovic, M, de Vos, W M (2008). High-throughput diversity and functionality analysis of the gastrointestinal tract microbiota. Gut 57: 1605-1615 [Abstract] [Full Text]  
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