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Applied and Environmental Microbiology, March 2007, p. 1452-1456, Vol. 73, No. 5
0099-2240/07/$08.00+0     doi:10.1128/AEM.02399-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Integrated Real-Time PCR for Detection and Monitoring of Legionella pneumophila in Water Systems{triangledown}

Diaraf Farba Yaradou,1 Sylvie Hallier-Soulier,2 Sophie Moreau,3 Florence Poty,3 Yves Hillion,2 Monique Reyrolle,1 Janine André,4 Gabriel Festoc,2 Karine Delabre,3 François Vandenesch,1 Jerome Etienne,1 and Sophie Jarraud1*

INSERM E0230, Lyon F-69008, France, and Université Lyon 1, Centre National de Référence des Legionella, Faculté Laennec, Lyon F-69008, France,1 GeneSystems, 1 rue du Courtil, Centre d'Affaires CICEA, Bâtiment 1, 35170 Bruz, France,2 CAE, Veolia Environnement, 1 place de Turenne, 94417 Saint Maurice, France,3 CARSO-Laboratoire Santé Environnement Hygiène de Lyon (CARSO-LSEHL), 321 avenue Jean-Jaurès, 69362 Lyon cedex 07, France4

Received 11 October 2006/ Accepted 19 December 2006

We evaluated a ready-to-use real-time quantitative Legionella pneumophila PCR assay system by testing 136 hot-water-system samples collected from 55 sites as well as 49 cooling tower samples collected from 20 different sites, in parallel with the standard culture method. The PCR assay was reproducible and suitable for routine quantification of L. pneumophila. An acceptable correlation between PCR and culture results was obtained for sanitary hot-water samples but not for cooling tower samples. We also monitored the same L. pneumophila-contaminated cooling tower for 13 months by analyzing 104 serial samples. The culture and PCR results were extremely variable over time, but the curves were similar. The differences between the PCR and culture results did not change over time and were not affected by regular biocide treatment. This ready-to-use PCR assay for L. pneumophila quantification could permit more timely disinfection of cooling towers.

* Corresponding author. Mailing address: Centre National de Référence des Légionelles, INSERM E-0230, Faculté de Médecine Laennec, Laboratoire de Bactériologie, 7 rue Guillaume-Paradin, 69372 Lyon cedex 08, France. Phone: (33) 47 877 86 42. Fax: (33) 47 877 86 58. E-mail: sophie.jarraud{at}univ-lyon1.fr.

{triangledown} Published ahead of print on 28 December 2006.

Applied and Environmental Microbiology, March 2007, p. 1452-1456, Vol. 73, No. 5
0099-2240/07/$08.00+0     doi:10.1128/AEM.02399-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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