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Applied and Environmental Microbiology, March 2007, p. 1676-1677, Vol. 73, No. 5
0099-2240/07/$08.00+0     doi:10.1128/AEM.01994-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Quantitative Assessment of the Tetracycline Resistance Gene Pool in Cheese Samples by Real-Time TaqMan PCR{triangledown}

Michele Y. Manuzon,1 Scott E. Hanna,1,{dagger} Hongliang Luo,1 Zhongtang Yu,3 W. James Harper,1 and Hua H. Wang1,2*

Department of Food Science and Technology,1 Department of Microbiology,2 Department of Animal Sciences, The Ohio State University, Columbus, Ohio 432103

Received 22 August 2006/ Accepted 4 January 2007

A TaqMan real-time PCR assay was developed to quantify the tetS gene pool present in retail cheeses. This protocol offers a rapid, specific, sensitive, and culture-independent method for assessing antibiotic resistance genes in food samples rich in fats and proteins.

* Corresponding author. Mailing address: Department of Food Science and Technology, The Ohio State University, Parker FST Building, 2015 Fyffe Ct., Columbus, OH 43210-1007. Phone: (614) 292-0579. Fax: (614) 292-0218. E-mail: wang.707{at}osu.edu.

{triangledown} Published ahead of print on 5 January 2007.

{dagger} Present address: Department of Defense Veterinary Food Analysis and Diagnostic Laboratory, 2472 Schofield Rd., Fort Sam Houston, San Antonio, TX 78234.

Applied and Environmental Microbiology, March 2007, p. 1676-1677, Vol. 73, No. 5
0099-2240/07/$08.00+0     doi:10.1128/AEM.01994-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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