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Applied and Environmental Microbiology, March 2007, p. 1678-1682, Vol. 73, No. 5
0099-2240/07/$08.00+0     doi:10.1128/AEM.02031-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Characterization of ISXax1, a Novel Insertion Sequence Restricted to Xanthomonas axonopodis pv. phaseoli (Variants fuscans and non-fuscans) and Xanthomonas axonopodis pv. vesicatoria{triangledown}

Seyed Mehdi Alavi,1 Stéphane Poussier,2 and Charles Manceau1*

UMR PaVé, INRA, 42 rue Georges Morel, 49071 Beaucouzé Cedex 01,1 INH, 2 rue Le Nôtre, 49045 Angers Cedex 01, France2

Received 28 August 2006/ Accepted 18 December 2006

ISXax1 is a novel insertion sequence belonging to the IS256 and Mutator families. Dot blot, Southern blot, and PCR analyses revealed that ISXax1 is restricted to Xanthomonas axonopodis pv. phaseoli (variants fuscans and non-fuscans) and X. axonopodis pv. vesicatoria strains. Directed AFLP also showed that a high degree of polymorphism is associated with ISXax1 insertion in these strains.

* Corresponding author. Mailing address: UMR PaVé, centre INRA, 42 rue Georges Morel, 49071 Beaucouzé Cedex 1, France. Phone: (33) 241 22 57 40. Fax: (33) 241 22 57 05. E-mail: manceau{at}angers.inra.fr.

{triangledown} Published ahead of print on 5 January 2007.

Applied and Environmental Microbiology, March 2007, p. 1678-1682, Vol. 73, No. 5
0099-2240/07/$08.00+0     doi:10.1128/AEM.02031-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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