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Applied and Environmental Microbiology, March 2007, p. 2009-2012, Vol. 73, No. 6
0099-2240/07/$08.00+0     doi:10.1128/AEM.02561-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Development of a Semiquantitative Degenerate Real-Time PCR-Based Assay for Estimation of Numbers of Butyryl-Coenzyme A (CoA) CoA Transferase Genes in Complex Bacterial Samples{triangledown}

Petra Louis* and Harry J. Flint

Microbial Ecology Group, Gut Health Division, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, United Kingdom

Received 2 November 2006/ Accepted 15 January 2007

We describe a new degenerate real-time PCR approach to simultaneously quantify phylogenetically different butyrate-producing bacteria based on the detection of butyryl-coenzyme A (CoA) CoA transferase genes. This pathway is present in numerically important groups of butyrate producers within the human colon, and thus this assay estimates the butyrate-producing ability of the microbiota.

* Corresponding author. Mailing address: Microbial Ecology Group, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, United Kingdom. Phone: 44 1224 712751. Fax: 44 1224 716687. E-mail: p.louis{at}rowett.ac.uk.

{triangledown} Published ahead of print on 26 January 2007.

Applied and Environmental Microbiology, March 2007, p. 2009-2012, Vol. 73, No. 6
0099-2240/07/$08.00+0     doi:10.1128/AEM.02561-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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