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Applied and Environmental Microbiology, April 2007, p. 2048-2053, Vol. 73, No. 7
0099-2240/07/$08.00+0     doi:10.1128/AEM.02500-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Antisense-RNA-Mediated Decreased Synthesis of Small, Acid-Soluble Spore Proteins Leads to Decreased Resistance of Clostridium perfringens Spores to Moist Heat and UV Radiation{triangledown}

Deepa Raju,1,2 Peter Setlow,3 and Mahfuzur R. Sarker1,2*

Department of Biomedical Sciences, College of Veterinary Medicine,1 Department of Microbiology, College of Science, Oregon State University, Corvallis, Oregon 97331,2 Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut 060303

Received 25 October 2006/ Accepted 21 January 2007

Previous work has suggested that a group of {alpha}/ß-type small, acid-soluble spore proteins (SASP) is involved in the resistance of Clostridium perfringens spores to moist heat. However, this suggestion is based on the analysis of C. perfringens spores lacking only one of the three genes encoding {alpha}/ß-type SASP in this organism. We have now used antisense RNA to decrease levels of {alpha}/ß-type SASP in C. perfringens spores by ~90%. These spores had significantly reduced resistance to both moist heat and UV radiation but not to dry heat. These results clearly demonstrate the important role of {alpha}/ß-type SASP in the resistance of C. perfringens spores.

* Corresponding author. Mailing address: Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, 216 Dryden Hall, Corvallis, OR 97331. Phone: (541) 737-6918. Fax: (541) 737-2730. E-mail: sarkerm{at}oregonstate.edu.

{triangledown} Published ahead of print on 26 January 2007.

Applied and Environmental Microbiology, April 2007, p. 2048-2053, Vol. 73, No. 7
0099-2240/07/$08.00+0     doi:10.1128/AEM.02500-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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