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Applied and Environmental Microbiology, April 2007, p. 2218-2223, Vol. 73, No. 7
0099-2240/07/$08.00+0     doi:10.1128/AEM.02024-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Comparison of Levels of Inactivation of Two Isolates of Giardia lamblia Cysts by UV Light{triangledown}

Dong Li,1 Stephen A. Craik,1* Daniel W. Smith,1 and Miodrag Belosevic2

Department of Civil & Environmental Engineering, University of Alberta, Edmonton, Alberta, Canada T6G 2W2,1 Department of Biological Science, University of Alberta, Edmonton, Alberta, Canada T6G 2E92

Received 25 August 2006/ Accepted 21 January 2007

The effects of 254-nm UV irradiation on two human isolates (WB and H3) of Giardia lamblia cysts were assessed using a collimated beam protocol and a Mongolian gerbil model. The levels of infection of cysts in the gerbils were assessed based on the presence of cysts in feces and the presence and activity of trophozoites in the small intestine of inoculated gerbils. The results suggest that there were differences in the infectivities of the WB and H3 isolates, as well as in susceptibilities of the parasites to UV light. Without UV exposure, gerbils were more readily infected by isolate H3 cysts. After UV exposure of the cysts, however, the gerbils were more susceptible to isolate WB cysts.


* Corresponding author. Mailing address: Department of Civil and Environmental Engineering, 3-133 Markin/CNRL Natural Resources Engineering Facility, University of Alberta, Edmonton, AB, Canada T6G 2W2. Phone: (780) 492-8548. Fax: (780) 492-8198. E-mail: scraik{at}ualberta.ca.

{triangledown} Published ahead of print on 2 February 2007.


Applied and Environmental Microbiology, April 2007, p. 2218-2223, Vol. 73, No. 7
0099-2240/07/$08.00+0     doi:10.1128/AEM.02024-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.