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Applied and Environmental Microbiology, April 2007, p. 2297-2305, Vol. 73, No. 7
0099-2240/07/$08.00+0     doi:10.1128/AEM.02193-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Campylobacter jejuni Strains Compete for Colonization in Broiler Chicks{triangledown}

Michael E. Konkel,1* Jeffrey E. Christensen,1 A. Singh Dhillon,2 Alison B. Lane,1 Rebekah Hare-Sanford,1 Dennis M. Schaberg,2 and Charles L. Larson1

School of Molecular Biosciences, Washington State University, Pullman, Washington,1 Avian Health and Food Safety Laboratory, College of Veterinary Medicine, Washington State University, Pullman, Washington2

Received 18 September 2006/ Accepted 28 January 2007

Campylobacter jejuni isolates possess multiple adhesive proteins termed adhesins, which promote the organism's attachment to epithelial cells. Based on the proposal that one or more adhesins are shared among C. jejuni isolates, we hypothesized that C. jejuni strains would compete for intestinal and cecal colonization in broiler chicks. To test this hypothesis, we selected two C. jejuni strains with unique SmaI pulsed-field gel electrophoresis macrorestriction profiles and generated one nalidixic acid-resistant strain (the F38011 Nalr strain) and one streptomycin-resistant strain (the 02-833L Strr strain). In vitro binding assays revealed that the C. jejuni F38011 Nalr and 02-833L Strr strains adhered to LMH chicken hepatocellular carcinoma epithelial cells and that neither strain influenced the binding potential of the other strain at low inoculation doses. However, an increase in the dose of the C. jejuni 02-833L Strr strain relative to that of the C. jejuni F38011 Nalr strain competitively inhibited the binding of the C. jejuni F38011 Nalr strain to LMH cells in a dose-dependent fashion. Similarly, the C. jejuni 02-833L Strr strain was found to significantly reduce the efficiency of intestinal and cecal colonization by the C. jejuni F38011 Nalr strain in broiler chickens. Based on the number of bacteria recovered from the ceca, the maximum number of bacteria that can colonize the digestive tracts of chickens may be limited by host constraints. Collectively, these data support the hypothesis that C. jejuni strains compete for colonization in chicks and suggest that it may be possible to design novel intervention strategies for reducing the level at which C. jejuni colonizes the cecum.

* Corresponding author. Mailing address: School of Molecular Biosciences, Washington State University, P.O. Box 644233, Pullman, WA 99164-4233. Phone: (509) 335-5039. Fax: (509) 335-1907. E-mail: konkel{at}wsu.edu.

{triangledown} Published ahead of print on 9 February 2007.

Applied and Environmental Microbiology, April 2007, p. 2297-2305, Vol. 73, No. 7
0099-2240/07/$08.00+0     doi:10.1128/AEM.02193-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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