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Applied and Environmental Microbiology, April 2007, p. 2333-2337, Vol. 73, No. 7
0099-2240/07/$08.00+0     doi:10.1128/AEM.01855-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Rapid Screening Method for Analyzing the Conjugated Linoleic Acid Production Capabilities of Bacterial Cultures{triangledown}

E. Barrett,1,2 R. P. Ross,1,2 G. F. Fitzgerald,2,3 and C. Stanton1,2*

Teagasc Biotechnology Centre, Moorepark Food Research Centre, Fermoy, County Cork, Ireland,1 Alimentary Pharmabiotic Centre, Biosciences Institute,2 Department of Microbiology, University College Cork, County Cork, Ireland3

Received 4 August 2006/ Accepted 20 January 2007

In this paper we describe a rapid method for identifying bacteria which convert free linoleic acid to conjugated linoleic acid (CLA). This method is based on spectrophotometric detection of CLA and compares well with the standard gas-liquid chromatography method. This method should facilitate high-throughput screening of bacterial isolates for the ability to produce conjugated fatty acids.

* Corresponding author. Mailing address: Teagasc Biotechnology Centre, Moorepark Food Research Centre, Fermoy, Co. Cork, Ireland. Phone: 353 (0) 25 42606. Fax: 353 (0) 25 42340. E-mail: catherine.stanton{at}teagasc.ie.

{triangledown} Published ahead of print on 2 February 2007.

Applied and Environmental Microbiology, April 2007, p. 2333-2337, Vol. 73, No. 7
0099-2240/07/$08.00+0     doi:10.1128/AEM.01855-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wall, R., Ross, R P., Shanahan, F., O'Mahony, L., O'Mahony, C., Coakley, M., Hart, O., Lawlor, P., Quigley, E. M, Kiely, B., Fitzgerald, G. F, Stanton, C. (2009). Metabolic activity of the enteric microbiota influences the fatty acid composition of murine and porcine liver and adipose tissues. Am. J. Clin. Nutr. 89: 1393-1401 [Abstract] [Full Text]  


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