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Applied and Environmental Microbiology, April 2007, p. 2338-2340, Vol. 73, No. 7
0099-2240/07/$08.00+0     doi:10.1128/AEM.01758-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Comparison of a Reporter Assay and Immunomagnetic Separation Real-Time Reverse Transcription-PCR for the Detection of Enteroviruses in Seeded Environmental Water Samples{triangledown},{dagger}

Yu-Chen Hwang,1 Oymon M. Leong,1 Wilfred Chen,2 and Marylynn V. Yates1*

Department of Environmental Sciences,1 Department of Chemical and Environmental Engineering, University of California, Riverside, California2

Received 25 July 2006/ Accepted 21 January 2007

Two newly developed protocols for infective virus detection were compared to the plaque assay. An immunomagnetic separation procedure coupled with real-time reverse transcription-PCR of viral nucleic acids was developed to identify intact enteroviral particles, and a reporter cell system responding to viral replication based on fluorescent resonance energy transfer for detection of infectious enteroviruses was tested. Both new procedures detected infective viruses in environmental samples at the same level as the plaque assay.

* Corresponding author. Mailing address: Department of Environmental Science, University of California, Riverside, CA 92521. Phone: (951) 827-2358. Fax: (951) 827-3993. E-mail: marylynn.yates{at}ucr.edu.

{triangledown} Published ahead of print on 2 February 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, April 2007, p. 2338-2340, Vol. 73, No. 7
0099-2240/07/$08.00+0     doi:10.1128/AEM.01758-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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