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Applied and Environmental Microbiology, April 2007, p. 2341-2343, Vol. 73, No. 7
0099-2240/07/$08.00+0     doi:10.1128/AEM.02519-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Water Transport in Intact Yeast Cells as Assessed by Fluorescence Self-Quenching{triangledown}

Graça Soveral,1,2* Ana Madeira,1 Maria C. Loureiro-Dias,3 and Teresa F. Moura1

REQUIMTE, Departamento Química, FCT-UNL, 2829-516 Caparica, Portugal,1 Faculdade de Farmácia, Universidade de Lisboa, 1649-003 Lisboa, Portugal,2 Instituto Superior de Agronomia, 1349-017 Lisboa, Portugal3

Received 29 October 2006/ Accepted 21 January 2007

Intact yeast cells loaded with 5- and-6-carboxyfluorescein were used to assess water transport. The results were similar to those previously reported for protoplasts assessed by using either fluorescence or light scattering, and the activation energies were 8.0 and 15.1 kcal mol–1 (33.4 and 63.2 kJ mol–1) for a strain overexpressing AQY1 aquaporin and a parental strain, respectively.

* Corresponding author. Mailing address: REQUIMTE, FCT/UNL, 2829-516 Caparica, Portugal. Phone: (351) 212948385. Fax: (351) 212948550. E-mail: soveral{at}dq.fct.unl.pt.

{triangledown} Published ahead of print on 2 February 2007.

Applied and Environmental Microbiology, April 2007, p. 2341-2343, Vol. 73, No. 7
0099-2240/07/$08.00+0     doi:10.1128/AEM.02519-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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