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Applied and Environmental Microbiology, April 2007, p. 2349-2353, Vol. 73, No. 7
0099-2240/07/$08.00+0     doi:10.1128/AEM.02880-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Effect of Lignocellulose-Derived Inhibitors on Growth of and Ethanol Production by Growth-Arrested Corynebacterium glutamicum R{triangledown}

Shinsuke Sakai,1 Yoshiki Tsuchida,1,2 Shohei Okino,1 Osamu Ichihashi,1 Hideo Kawaguchi,1 Takashi Watanabe,3 Masayuki Inui,1 and Hideaki Yukawa1*

Research Institute of Innovative Technology for the Earth, 9-2 Kizugawadai, Kizu-cho, Soraku-gun, Kyoto 619-0292, Japan,1 Honda R&D Co., Ltd., 1-4-1 Chuo Wako-shi, Saitama 351-0193, Japan,2 Laboratory of Biomass Conversion, Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan3

Received 13 December 2006/ Accepted 24 January 2007

In cellulosic ethanol production, pretreatment of a biomass to facilitate enzymatic hydrolysis inevitably yields fermentation inhibitors such as organic acids, furans, and phenols. With representative inhibitors included in the medium at various concentrations, individually or in various combinations, ethanol production by Corynebacterium glutamicum R under growth-arrested conditions was investigated. In the presence of various inhibitors, the 62 to 100% ethanol productivity retained by the C. glutamicum R-dependent method far exceeded that retained by previously reported methods.

* Corresponding author. Mailing address: Research Institute of Innovative Technology for the Earth, 9-2 Kizugawadai, Kizu-cho, Soraku-gun, Kyoto 619-0292, Japan. Phone: 81-774-75-2308. Fax: 81-774-75-2321. E-mail: mmg-lab{at}rite.or.jp.

{triangledown} Published ahead of print on 2 February 2007.

Applied and Environmental Microbiology, April 2007, p. 2349-2353, Vol. 73, No. 7
0099-2240/07/$08.00+0     doi:10.1128/AEM.02880-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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