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Applied and Environmental Microbiology, April 2007, p. 2364-2368, Vol. 73, No. 7
0099-2240/07/$08.00+0     doi:10.1128/AEM.00207-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Nickel-Resistant Determinant from Leptospirillum ferriphilum{triangledown}

Jian Tian,1 Ningfeng Wu,1* Jiang Li,2 Yajie Liu,2 Jun Guo,1 Bin Yao,3* and Yunliu Fan1

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China,1 East China Institute of Technology, Wuzhou, Jiangxi, China,2 Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China3

Received 26 January 2007/ Accepted 30 January 2007

Leptospirillum ferriphilum strain UBK03 isolated from a mine in Jiangxi, China, is resistant to Ni2+ (30 to 40 mM). A four-gene nickel resistance cluster was identified and, when transformed into Escherichia coli, enabled growth in 6 mM nickel. Mutation experiments revealed that the genes ncrA, ncrB, and ncrC could confer nickel resistance in Escherichia coli, whereas the gene ncrY could have a negative effect on nickel resistance.

* Corresponding author. Mailing address for Ningfeng Wu: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing 100081, China. Phone: 86-10-68919864. Fax: 86-10-62136981. E-mail: wunf{at}caas.net.cn. Mailing address for Bin Yao: Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Phone and fax: 86-10-68975126. E-mail: yaobin{at}mail.caas.net.cn.

{triangledown} Published ahead of print on 9 February 2007.

Applied and Environmental Microbiology, April 2007, p. 2364-2368, Vol. 73, No. 7
0099-2240/07/$08.00+0     doi:10.1128/AEM.00207-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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