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Applied and Environmental Microbiology, April 2007, p. 2373-2377, Vol. 73, No. 7
0099-2240/07/$08.00+0 doi:10.1128/AEM.02341-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Detection and Identification by PCR of a Highly Virulent Phylogenetic Subgroup among Extraintestinal Pathogenic Escherichia coli B2 Strains
Philippe Bidet,1
Arnaud Metais,2
Farah Mahjoub-Messai,1
Lionel Durand,2
Marie Dehem,3
Yannick Aujard,4
Edouard Bingen,1*
Xavier Nassif,2 and
Stéphane Bonacorsi1
Laboratoire d'Études de Génétique Bactérienne dans les Infections de l'Enfant (EA3105), Université Denis Diderot-Paris 7, Service de Microbiologie,1
Service de Néonatologie, Hôpital Robert Debré, Assistance Publique-Hôpitaux de Paris (Ap-Hp), ParisFrance;,4
INSERM U570, Faculté de Médecine Necker-Enfants Malades, Université René Descartes-Paris 5, Paris, France,2
Genoscreen, Lille, France3
Received 4 October 2006/
Accepted 27 January 2007
Closely related Escherichia coli B2 strains O1:K1, O2:K1, O18:K1, and O45:K1 constitute a major subgroup causing extraintestinal infections. A DNA pathoarray analysis was used to develop a PCR specific for this subgroup that was included in the multiplex phylogenetic-grouping PCR method. Our PCR may serve to identify this virulent subgroup among different ecological niches.
* Corresponding author. Mailing address: Service de Microbiologie, Hôpital Robert Debré, 48 Bd Sérurier, 75395 Paris cedex 19, France. Phone: 33 1 40 03 23 40. Fax: 33 1 40 03 24 50. E-mail:
edouard.bingen{at}rdb.ap-hop-paris.fr.
Published ahead of print on 9 February 2007.
Applied and Environmental Microbiology, April 2007, p. 2373-2377, Vol. 73, No. 7
0099-2240/07/$08.00+0 doi:10.1128/AEM.02341-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.
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