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Applied and Environmental Microbiology, April 2007, p. 2373-2377, Vol. 73, No. 7
0099-2240/07/$08.00+0     doi:10.1128/AEM.02341-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Detection and Identification by PCR of a Highly Virulent Phylogenetic Subgroup among Extraintestinal Pathogenic Escherichia coli B2 Strains

Laboratoire d'Études de Génétique Bactérienne dans les Infections de l'Enfant (EA3105), Université Denis Diderot-Paris 7, Service de Microbiologie,¹ Service de Néonatologie, Hôpital Robert Debré, Assistance Publique-Hôpitaux de Paris (Ap-Hp), ParisFrance;,⁴ INSERM U570, Faculté de Médecine Necker-Enfants Malades, Université René Descartes-Paris 5, Paris, France,² Genoscreen, Lille, France³

Received 4 October 2006/ Accepted 27 January 2007

Closely related Escherichia coli B2 strains O1:K1, O2:K1, O18:K1, and O45:K1 constitute a major subgroup causing extraintestinal infections. A DNA pathoarray analysis was used to develop a PCR specific for this subgroup that was included in the multiplex phylogenetic-grouping PCR method. Our PCR may serve to identify this virulent subgroup among different ecological niches.

Published ahead of print on 9 February 2007.

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