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Applied and Environmental Microbiology, April 2007, p. 2390-2393, Vol. 73, No. 7
0099-2240/07/$08.00+0     doi:10.1128/AEM.02266-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

A Derepression System Based on the Bacillus subtilis Sporulation Pathway Offers Dynamic Control of Heterologous Gene Expression{triangledown}

Reindert Nijland,{dagger} Jan-Willem Veening,{dagger} and Oscar P. Kuipers*

Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands

Received 26 September 2006/ Accepted 30 January 2007

By rewiring the sporulation gene-regulatory network of Bacillus subtilis, we generated a novel expression system relying on derepression. The gene of interest is placed under the control of the abrB promoter, which is active only when Spo0A is absent, and Spo0A is controlled via an IPTG (isopropyl-ß-D-thiogalactopyranoside)-inducible promoter.

* Corresponding author. Mailing address: Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands. Phone: 31 50 3632093. Fax: 31 50 3632348. E-mail: o.p.kuipers{at}rug.nl.

{triangledown} Published ahead of print on 9 February 2007.

{dagger} Both authors contributed equally.

Applied and Environmental Microbiology, April 2007, p. 2390-2393, Vol. 73, No. 7
0099-2240/07/$08.00+0     doi:10.1128/AEM.02266-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lulko, A. T., Veening, J.-W., Buist, G., Smits, W. K., Blom, E. J., Beekman, A. C., Bron, S., Kuipers, O. P. (2007). Production and Secretion Stress Caused by Overexpression of Heterologous {alpha}-Amylase Leads to Inhibition of Sporulation and a Prolonged Motile Phase in Bacillus subtilis. Appl. Environ. Microbiol. 73: 5354-5362 [Abstract] [Full Text]  


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