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Applied and Environmental Microbiology, April 2007, p. 2458-2467, Vol. 73, No. 8
0099-2240/07/$08.00+0     doi:10.1128/AEM.02201-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Genotypic and Physiological Characterization of Saccharomyces boulardii, the Probiotic Strain of Saccharomyces cerevisiae{triangledown}

Laura Edwards-Ingram,1 Paul Gitsham,1 Nicola Burton,1 Geoff Warhurst,2 Ian Clarke,1 David Hoyle,3 Stephen G. Oliver,1 and Lubomira Stateva1*

Faculty of Life Sciences, The University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, United Kingdom,1 Gut Barrier Research Group, Clinical Sciences, Hope Hospital, Salford M6 8HD, United Kingdom,2 North West Institute for Bio-Health Informatics, School of Medicine, The University of Manchester, Stopford Building, Oxford Road, Manchester M13 9PT, United Kingdom3

Received 19 September 2006/ Accepted 22 January 2007

Saccharomyces boulardii, a yeast that was isolated from fruit in Indochina, has been used as a remedy for diarrhea since 1950 and is now a commercially available treatment throughout Europe, Africa, and South America. Though initially classified as a separate species of Saccharomyces, recent publications have shown that the genome of S. boulardii is so similar to Saccharomyces cerevisiae that the two should be classified as conspecific. This raises the question of the distinguishing molecular and phenotypic characteristics present in S. boulardii that make it perform more effectively as a probiotic organism compared to other strains of S. cerevisiae. This investigation reports some of these distinguishing characteristics including enhanced ability for pseudohyphal switching upon nitrogen limitation and increased resistance to acidic pH. However, these differences did not correlate with increased adherence to epithelial cells or transit through mouse gut. Pertinent characteristics of the S. boulardii genome such as trisomy of chromosome IX, altered copy number of a number of individual genes, and sporulation deficiency have been revealed by comparative genome hybridization using oligonucleotide-based microarrays coupled with a rigorous statistical analysis. The contributions of the different genomic and phenotypic features of S. boulardii to its probiotic nature are discussed.

* Corresponding author. Mailing address: Faculty of Life Sciences, The University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, United Kingdom. Phone: 44 161 306 4208. Fax: 44 161 275 5082. E-mail: l.stateva{at}manchester.ac.uk

{triangledown} Published ahead of print on 9 February 2007.

Applied and Environmental Microbiology, April 2007, p. 2458-2467, Vol. 73, No. 8
0099-2240/07/$08.00+0     doi:10.1128/AEM.02201-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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