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Applied and Environmental Microbiology, April 2007, p. 2708-2717, Vol. 73, No. 8
0099-2240/07/$08.00+0     doi:10.1128/AEM.02140-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Isolation and Characterization of Soil Bacteria That Define Terriglobus gen. nov., in the Phylum Acidobacteria{triangledown}

Stephanie A. Eichorst, John A. Breznak, and Thomas M. Schmidt*

Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan

Received 11 September 2006/ Accepted 5 February 2007

Bacteria in the phylum Acidobacteria are widely distributed and abundant in soils, but their ecological roles are poorly understood, owing in part to a paucity of cultured representatives. In a molecular survey of acidobacterial diversity at the Michigan State University Kellogg Biological Station Long-Term Ecological Research site, 27% of acidobacterial 16S rRNA gene clones in a never-tilled, successional plant community belonged to subdivision 1, whose relative abundance varied inversely with soil pH. Strains of subdivision 1 were isolated from these never-tilled soils using low-nutrient medium incubated for 3 to 4 weeks under elevated levels of carbon dioxide, which resulted in a slightly acidified medium that matched the pH optima of the strains (between 5 and 6). Colonies were approximately 1 mm in diameter and either white or pink, the latter due to a carotenoid(s) that was synthesized preferentially under 20% instead of 2% oxygen. Strains were gram-negative, aerobic, chemo-organotrophic, nonmotile rods that produced an extracellular matrix. All strains contained either one or two copies of the 16S rRNA encoding gene, which along with a relatively slow doubling time (10 to 15 h at ca. 23°C) is suggestive of an oligotrophic lifestyle. Six of the strains are sufficiently similar to one another, but distinct from previously named Acidobacteria, to warrant creation of a new genus, Terriglobus, with Terriglobus roseus defined as the type species. The physiological and nutritional characteristics of Terriglobus are consistent with its potential widespread distribution in soil.

* Corresponding author. Mailing address: Michigan State University, Department of Microbiology and Molecular Genetics, 6180 Biomedical and Physical Sciences Building, East Lansing, MI 48824-4320. Phone: (517) 355-6463, ext. 1606. Fax: (517) 353-8957. E-mail: tschmidt{at}msu.edu

{triangledown} Published ahead of print on 9 February 2007.

Applied and Environmental Microbiology, April 2007, p. 2708-2717, Vol. 73, No. 8
0099-2240/07/$08.00+0     doi:10.1128/AEM.02140-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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