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Applied and Environmental Microbiology, April 2007, p. 2708-2717, Vol. 73, No. 8
0099-2240/07/$08.00+0 doi:10.1128/AEM.02140-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Isolation and Characterization of Soil Bacteria That Define Terriglobus gen. nov., in the Phylum Acidobacteria
Stephanie A. Eichorst,
John A. Breznak, and
Thomas M. Schmidt*
Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan
Received 11 September 2006/
Accepted 5 February 2007
Bacteria in the phylum Acidobacteria are widely distributed and abundant in soils, but their ecological roles are poorly understood, owing in part to a paucity of cultured representatives. In a molecular survey of acidobacterial diversity at the Michigan State University Kellogg Biological Station Long-Term Ecological Research site, 27% of acidobacterial 16S rRNA gene clones in a never-tilled, successional plant community belonged to subdivision 1, whose relative abundance varied inversely with soil pH. Strains of subdivision 1 were isolated from these never-tilled soils using low-nutrient medium incubated for 3 to 4 weeks under elevated levels of carbon dioxide, which resulted in a slightly acidified medium that matched the pH optima of the strains (between 5 and 6). Colonies were approximately 1 mm in diameter and either white or pink, the latter due to a carotenoid(s) that was synthesized preferentially under 20% instead of 2% oxygen. Strains were gram-negative, aerobic, chemo-organotrophic, nonmotile rods that produced an extracellular matrix. All strains contained either one or two copies of the 16S rRNA encoding gene, which along with a relatively slow doubling time (10 to 15 h at ca. 23°C) is suggestive of an oligotrophic lifestyle. Six of the strains are sufficiently similar to one another, but distinct from previously named Acidobacteria, to warrant creation of a new genus, Terriglobus, with Terriglobus roseus defined as the type species. The physiological and nutritional characteristics of Terriglobus are consistent with its potential widespread distribution in soil.
* Corresponding author. Mailing address: Michigan State University, Department of Microbiology and Molecular Genetics, 6180 Biomedical and Physical Sciences Building, East Lansing, MI 48824-4320. Phone: (517) 355-6463, ext. 1606. Fax: (517) 353-8957. E-mail:
tschmidt{at}msu.edu
Published ahead of print on 9 February 2007.
Applied and Environmental Microbiology, April 2007, p. 2708-2717, Vol. 73, No. 8
0099-2240/07/$08.00+0 doi:10.1128/AEM.02140-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.
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