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Applied and Environmental Microbiology, April 2007, p. 2758-2761, Vol. 73, No. 8
0099-2240/07/$08.00+0     doi:10.1128/AEM.02844-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

A mariner-Based Transposition System for Listeria monocytogenes{triangledown}

Min Cao,{dagger} Alan Pavinski Bitar, and Hélène Marquis*

Department of Microbiology and Immunology, Cornell University, Ithaca, New York 14853

Received 6 December 2006/ Accepted 12 February 2007

In this study, we developed a new mariner-based transposition system for Listeria monocytogenes. The mariner-based system has a high rate of transposition and a low rate of plasmid retention, and transposition is very random, making it an ideal tool for high-throughput transposon mutagenesis in L. monocytogenes.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, VMC C5-169, Cornell University, Ithaca, NY 14853-6401. Phone: (607) 253-3273. Fax: (607) 253-3384. E-mail: hm72{at}cornell.edu

{triangledown} Published ahead of print on 16 February 2007.

{dagger} Present address: Department of Biological Sciences, Clemson University, Clemson, SC 29634.

Applied and Environmental Microbiology, April 2007, p. 2758-2761, Vol. 73, No. 8
0099-2240/07/$08.00+0     doi:10.1128/AEM.02844-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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