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Applied and Environmental Microbiology, May 2007, p. 3084-3090, Vol. 73, No. 9
0099-2240/07/$08.00+0     doi:10.1128/AEM.02893-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Adherence of Enterohemorrhagic Escherichia coli O157, O26, and O111 Strains to Bovine Intestinal Explants Ex Vivo{triangledown}

Francis Girard,1 Francis Dziva,2 Pauline van Diemen,2 Alan D. Phillips,3 Mark P. Stevens,2* and Gad Frankel1

Division of Cell and Molecular Biology, Imperial College London, London,1 Enteric Bacterial Pathogens Group, Division of Microbiology, Institute for Animal Health, Compton, Berkshire,2 Centre for Paediatric Gastroenterology, Royal Free Hospital, London, United Kingdom3

Received 14 December 2006/ Accepted 26 February 2007

We used bovine intestinal organ culture to study infection by enterohemorrhagic Escherichia coli serogroups O157, O26, and O111. We show colonization and attaching and effacing lesion formation on explants derived from the ileum, colon, and rectum. Intimin and Tir were detected at the sites of adherent bacteria; Tir was essential for colonization.

* Corresponding author. Mailing address: Enteric Bacterial Pathogens Group, Division of Microbiology, Institute for Animal Health, Compton Laboratory, Compton, Berkshire RG20 7NN, United Kingdom. Phone: 44 (0)1635 577915. Fax: 44 (0)1635 577237. E-mail: mark-p.stevens{at}bbsrc.ac.uk

{triangledown} Published ahead of print on 9 March 2007.

Applied and Environmental Microbiology, May 2007, p. 3084-3090, Vol. 73, No. 9
0099-2240/07/$08.00+0     doi:10.1128/AEM.02893-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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