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Applied and Environmental Microbiology, May 2007, p. 3105-3108, Vol. 73, No. 9
0099-2240/07/$08.00+0     doi:10.1128/AEM.02765-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Bacterial Degradation of N,N-Diethyl-m-Toluamide (DEET): Cloning and Heterologous Expression of DEET Hydrolase{triangledown}

Giomar Rivera-Cancel,1 Daniela Bocioaga,1 and Anthony G. Hay1,2*

Department of Microbiology,1 Institute for Comparative and Environmental Toxicology, Cornell University, Ithaca, New York 148532

Received 27 November 2006/ Accepted 26 February 2007

Pseudomonas putida DTB grew aerobically with N,N-diethyl-m-toluamide (DEET) as a sole carbon source, initially breaking it down into 3-methylbenzoate and diethylamine. The former was further metabolized via 3-methylcatechol and meta ring cleavage. A gene from DTB, dthA, was heterologously expressed and shown to encode the ability to hydrolyze DEET into 3-methylbenzoate and diethylamine.

* Corresponding author. Mailing address: Department of Microbiology, Cornell University, Ithaca, NY 14853. Phone: (607) 255-8471. Fax: (607) 255-3904. E-mail: agh5{at}cornell.edu

{triangledown} Published ahead of print on 2 March 2007.

Applied and Environmental Microbiology, May 2007, p. 3105-3108, Vol. 73, No. 9
0099-2240/07/$08.00+0     doi:10.1128/AEM.02765-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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