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Applied and Environmental Microbiology, May 2007, p. 3113-3116, Vol. 73, No. 9
0099-2240/07/$08.00+0     doi:10.1128/AEM.02012-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Acidobacteria Phylum Sequences in Uranium-Contaminated Subsurface Sediments Greatly Expand the Known Diversity within the Phylum{triangledown} ,{dagger}

Susan M. Barns, Elizabeth C. Cain, Leslie Sommerville,{ddagger} and Cheryl R. Kuske*

Bioscience Division, M888, Los Alamos National Laboratory, Los Alamos, New Mexico 87545

Received 24 August 2006/ Accepted 22 February 2007

The abundance and composition of bacteria of the phylum Acidobacteria were surveyed in subsurface sediments from uranium-contaminated sites using amplification of 16S rRNA genes followed by clone/sequence analysis. Analysis of sequences from this study and public databases produced a revised and greatly expanded phylogeny of the Acidobacteria phylum consisting of 26 subgroups.

* Corresponding author. Mailing address: Bioscience Division, M888, Los Alamos National Laboratory, Los Alamos, NM 87545. Phone: (505) 665-4800. Fax: (505) 665-3024. E-mail: kuske{at}lanl.gov

{triangledown} Published ahead of print on 2 March 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

{ddagger} Present address: Chemistry Department, Ft. Lewis College, Durango, CO 81301.

Applied and Environmental Microbiology, May 2007, p. 3113-3116, Vol. 73, No. 9
0099-2240/07/$08.00+0     doi:10.1128/AEM.02012-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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