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Applied and Environmental Microbiology, January 2008, p. 305-308, Vol. 74, No. 1
0099-2240/08/$08.00+0     doi:10.1128/AEM.01793-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Cloning of a Novel Gene Encoding β-1,3-Xylosidase from a Marine Bacterium, Vibrio sp. Strain XY-214, and Characterization of the Gene Product{triangledown}

Yoshiaki Umemoto, Ryosuke Onishi, and Toshiyoshi Araki*

Graduate School of Bioresources, Mie University, 1577 Kurimamachiya, Tsu, Mie 514-8507, Japan

Received 2 August 2007/ Accepted 16 October 2007

The β-1,3-xylosidase gene (xloA) of Vibrio sp. strain XY-214 was cloned and expressed in Escherichia coli. The xloA gene consisted of a 1,608-bp nucleotide sequence encoding a protein of 535 amino acids with a predicted molecular weight of 60,835. The recombinant β-1,3-xylosidase hydrolyzed β-1,3-xylooligosaccharides to D-xylose as a final product.

* Corresponding author. Mailing address: Graduate School of Bioresources, Mie University, 1577 Kurimamachiya, Tsu, Mie 514-8507, Japan. Phone: 81-59-231-9561. Fax: 81-59-231-9540. E-mail: araki{at}bio.mie-u.ac.jp

{triangledown} Published ahead of print on 9 November 2007.

Applied and Environmental Microbiology, January 2008, p. 305-308, Vol. 74, No. 1
0099-2240/08/$08.00+0     doi:10.1128/AEM.01793-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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