This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mojica, E.-R. E.
Right arrow Articles by Aga, D. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mojica, E.-R. E.
Right arrow Articles by Aga, D. S.
Agricola
Right arrow Articles by Mojica, E.-R. E.
Right arrow Articles by Aga, D. S.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, January 2008, p. 323-326, Vol. 74, No. 1
0099-2240/08/$08.00+0     doi:10.1128/AEM.01407-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Formation of N-Ethylmaleimide (NEM)-Glutathione Conjugate and N-Ethylmaleamic Acid Revealed by Mass Spectral Characterization of Intracellular and Extracellular Microbial Metabolites of NEM{triangledown}

Elmer-Rico E. Mojica, Sungpyo Kim, and Diana S. Aga*

Department of Chemistry, Natural Sciences Complex, University at Buffalo, The State University of New York, Buffalo, New York 14260-3000

Received 25 June 2007/ Accepted 22 October 2007

The extracellular and intracellular metabolites formed upon exposure of activated sludge microorganisms to a sublethal concentration of N-ethylmaleimide were monitored by liquid chromatography with ion trap mass spectrometry. The metabolite N-ethylsuccinimido-S-glutathione (m/z 433) was converted rapidly to N-(2-oxoethyl)-2,2-(propionylamino)propanamide (m/z 187) and N-ethylmaleamic acid (m/z 144).

* Corresponding author. Mailing address: Department of Chemistry, Natural Sciences Complex, University at Buffalo, The State University of New York, Buffalo, NY 14260-3000. Phone: (716) 645-6800. Fax: (716) 645-6963. E-mail: dianaaga{at}buffalo.edu

{triangledown} Published ahead of print on 2 November 2007.

Applied and Environmental Microbiology, January 2008, p. 323-326, Vol. 74, No. 1
0099-2240/08/$08.00+0     doi:10.1128/AEM.01407-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»