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Applied and Environmental Microbiology, January 2008, p. 329-332, Vol. 74, No. 1
0099-2240/08/$08.00+0     doi:10.1128/AEM.01448-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Eucaryotic Diversity in a Hypersaline Microbial Mat{triangledown} ,{dagger}

Leah M. Feazel,1,{ddagger} John R. Spear,2,{ddagger} Alicia B. Berger,3,{ddagger} J. Kirk Harris,1 Daniel N. Frank,1 Ruth E. Ley,4 and Norman R. Pace1*

Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347,1 Division of Environmental Science and Engineering, Colorado School of Mines, Golden, Colorado 80401,2 Cancer Biology Program and Pathology Department, School of Medicine, Stanford University, Palo Alto, California 94305,3 School of Medicine, Washington University, St. Louis, Missouri 631084

Received 28 June 2007/ Accepted 25 October 2007

To determine the eucaryotic diversity of the hypersaline Guerrero Negro microbial mat, we amplified 18S rRNA genes from DNA extracted from this mat and constructed and analyzed clone libraries. The extent of eucaryotic diversity detected was remarkably low, only 15 species among 890 clones analyzed. Six eucaryotic kingdoms were represented, as well as a novel cluster of sequences. Nematode sequences dominated the clone libraries.

* Corresponding author. Mailing address: Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 90309-0347. Phone: (303) 735-1864. Fax: (303) 492-7744. E-mail: nrpace{at}colorado.edu

{triangledown} Published ahead of print on 9 November 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

{ddagger} These authors contributed equally to this work.

Applied and Environmental Microbiology, January 2008, p. 329-332, Vol. 74, No. 1
0099-2240/08/$08.00+0     doi:10.1128/AEM.01448-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Boyd, E. S., Spear, J. R., Peters, J. W. (2009). [FeFe] Hydrogenase Genetic Diversity Provides Insight into Molecular Adaptation in a Saline Microbial Mat Community. Appl. Environ. Microbiol. 75: 4620-4623 [Abstract] [Full Text]  
  • Robertson, C. E., Spear, J. R., Harris, J. K., Pace, N. R. (2009). Diversity and Stratification of Archaea in a Hypersaline Microbial Mat. Appl. Environ. Microbiol. 75: 1801-1810 [Abstract] [Full Text]  


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