This Article Full Text Full Text (PDF) Supplemental material Other Versions of this Article: AEM.01448-07v1 74/1/329    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Google Scholar Articles by Feazel, L. M. Articles by Pace, N. R. PubMed PubMed Citation Articles by Feazel, L. M. Articles by Pace, N. R. Agricola Articles by Feazel, L. M. Articles by Pace, N. R.

Eucaryotic Diversity in a Hypersaline Microbial Mat ^,

Leah M. Feazel,^1, John R. Spear,^2, Alicia B. Berger,^3, J. Kirk Harris,¹ Daniel N. Frank,¹ Ruth E. Ley,⁴ and Norman R. Pace^1*

Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347,¹ Division of Environmental Science and Engineering, Colorado School of Mines, Golden, Colorado 80401,² Cancer Biology Program and Pathology Department, School of Medicine, Stanford University, Palo Alto, California 94305,³ School of Medicine, Washington University, St. Louis, Missouri 63108⁴

Received 28 June 2007/ Accepted 25 October 2007

To determine the eucaryotic diversity of the hypersaline Guerrero Negro microbial mat, we amplified 18S rRNA genes from DNA extracted from this mat and constructed and analyzed clone libraries. The extent of eucaryotic diversity detected was remarkably low, only 15 species among 890 clones analyzed. Six eucaryotic kingdoms were represented, as well as a novel cluster of sequences. Nematode sequences dominated the clone libraries.

Published ahead of print on 9 November 2007.

Supplemental material for this article may be found at http://aem.asm.org/.

These authors contributed equally to this work.