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Applied and Environmental Microbiology, May 2008, p. 2940-2949, Vol. 74, No. 10
0099-2240/08/$08.00+0     doi:10.1128/AEM.01156-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Unexpected Importance of Potential Parasites in the Composition of the Freshwater Small-Eukaryote Community{triangledown}

Cécile Lepère,1 Isabelle Domaizon,2 and Didier Debroas1*

Université Blaise Pascal, Laboratoire de Biologie des Protistes, UMR CNRS 6023, 63177 Aubière, France,1 Université de Savoie, UMR CARRTEL, 73376 Le Bourget du Lac, France2

Received 23 May 2007/ Accepted 7 March 2008

The diversity of small eukaryotes (0.2 to 5 µm) in a mesotrophic lake (Lake Bourget) was investigated using 18S rRNA gene library construction and fluorescent in situ hybridization coupled with tyramide signal amplification (TSA-FISH). Samples collected from the epilimnion on two dates were used to extend a data set previously obtained using similar approaches for lakes with a range of trophic types. A high level of diversity was recorded for this system with intermediate trophic status, and the main sequences from Lake Bourget were affiliated with ciliates (maximum, 19% of the operational taxonomic units [OTUs]), cryptophytes (33%), stramenopiles (13.2%), and cercozoa (9%). Although the comparison of TSA-FISH results and clone libraries suggested that the level of Chlorophyceae may have been underestimated using PCR with 18S rRNA primers, heterotrophic organisms dominated the small-eukaryote assemblage. We found that a large fraction of the sequences belonged to potential parasites of freshwater phytoplankton, including sequences affiliated with fungi and Perkinsozoa. On average, these sequences represented 30% of the OTUs (40% of the clones) obtained for each of two dates for Lake Bourget. Our results provide information on lacustrine small-eukaryote diversity and structure, adding to the phylogenetic data available for lakes with various trophic types.

* Corresponding author. Mailing address: Université Blaise Pascal, Laboratoire de Biologie des Protistes, UMR CNRS 6023, 63117 Aubière, France. Phone: 33 473407837. Fax: 33 473407670. E-mail: didier.debras{at}free.fr

{triangledown} Published ahead of print on 21 March 2008.

Applied and Environmental Microbiology, May 2008, p. 2940-2949, Vol. 74, No. 10
0099-2240/08/$08.00+0     doi:10.1128/AEM.01156-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mangot, J.-F., Lepere, C., Bouvier, C., Debroas, D., Domaizon, I. (2009). Community Structure and Dynamics of Small Eukaryotes Targeted by New Oligonucleotide Probes: New Insight into the Lacustrine Microbial Food Web. Appl. Environ. Microbiol. 75: 6373-6381 [Abstract] [Full Text]  
  • Valster, R. M., Wullings, B. A., Bakker, G., Smidt, H., van der Kooij, D. (2009). Free-Living Protozoa in Two Unchlorinated Drinking Water Supplies, Identified by Phylogenic Analysis of 18S rRNA Gene Sequences. Appl. Environ. Microbiol. 75: 4736-4746 [Abstract] [Full Text]  


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